http://www.cnr.it/ontology/cnr/individuo/prodotto/ID9007
Ig from Trematomus bernacchii: a model for structural analysis of Ig from a cold adapted species. (Articolo in rivista)
- Type
- Label
- Ig from Trematomus bernacchii: a model for structural analysis of Ig from a cold adapted species. (Articolo in rivista) (literal)
- Anno
- 2000-01-01T00:00:00+01:00 (literal)
- Alternative label
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- Coscia MR, Alfieri V, Oreste U. (literal)
- Pagina inizio
- Pagina fine
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
- Note
- ISI Web of Science (WOS) (literal)
- Titolo
- Ig from Trematomus bernacchii: a model for structural analysis of Ig from a cold adapted species. (literal)
- Abstract
- The immunoglobulin of the Antarctic teleost Trematomus bernacchii was investigated with respect to the amino acid sequence of the heavy chain and the diversity of the gene segments encoding the variable domain. A cDNA expression library was constructed from the spleen and immunoscreened with rabbit anti-T. bernacchii IgH. A positive clone, found to encode the complete IgH sequence, including the leader peptide, was sequenced. Comparison of the deduced amino acid sequence of T. bernacchii IgH constant domains with those from other actinopterygians showed identities ranging between 53.1 and 71.1%. Multiple sequence alignment revealed the presence of two remarkable insertions occurring in the VH-CH1 switch region and at the CH2-CH3 boundary. Additional differences were found in the number and positions of putative N-glycosylation sites as well as of cysteine residues not involved in the intradomain bridges. An oligonucleotide primer was designed to amplify in PCR cDNA encoding different variable domains. The nucleotide sequence of 33 VH clones was determined, and found distributed over three gene families, named Trbe VH I, Trbe VH II, and Trbe VH III. Nine distinct JH gene segments were also identified. The differences found with respect to other fish Ig, as well as the low diversity found in VH genes, were attributed to adaptation processes. (literal)
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