http://www.cnr.it/ontology/cnr/individuo/prodotto/ID8989

Beta-glycosyl azides as substrates for a-glycosynthases: preparation of efficient alpha-L-fucosynthases (Articolo in rivista)

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Beta-glycosyl azides as substrates for a-glycosynthases: preparation of efficient alpha-L-fucosynthases (Articolo in rivista) (literal)

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Cobucci-Ponzano B.; Conte F.; Bedini E.; Corsaro M.M.; Parrilli M.; Sulzenbacher G.; Lipski A.; Dal Piaz F.; Lepore L.; Rossi M.; Moracci M. (2009)
Beta-glycosyl azides as substrates for a-glycosynthases: preparation of efficient alpha-L-fucosynthases
in Chemistry & biology (Print); Elsevier, Amsterdam (Paesi Bassi)
(literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

Cobucci-Ponzano B.; Conte F.; Bedini E.; Corsaro M.M.; Parrilli M.; Sulzenbacher G.; Lipski A.; Dal Piaz F.; Lepore L.; Rossi M.; Moracci M. (literal)

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Institute of Protein Biochemistry - Consiglio Nazionale delle Ricerche, Via P. Castellino 111, 80131, Naples, Italy Dipartimento di Chimica Organica e Biochimica, Universita` di Napoli Federico II, Complesso Universitario di Monte S. Angelo, via Cinthia 4, 80126 Napoli, Italy Architecture et Fonction des Macromole` cules Biologiques, UMR6098 CNRS, Universite` de Provence, Universite` de la Me` diterrane` e, Case 932, 163 Avenue de Luminy, 13288 Marseille Cedex 9, France Dipartimento di Scienze Farmaceutiche, Universita` di Salerno, Via Ponte Don Melillo, 84084 Fisciano, Salerno, Italy Dipartimento di Biologia Strutturale e Funzionale, Universita` di Napoli Federico II, Complesso Universitario di Monte S. Angelo, via Cinthia 4, 80126 Napoli, Italy (literal)

Titolo

Beta-glycosyl azides as substrates for a-glycosynthases: preparation of efficient alpha-L-fucosynthases (literal)

Abstract

Fucose-containingoligosaccharides play a central role in physio-pathological events, and fucosylated oligosaccharides have interesting potential applications in biomedicine. No methods for the large-scale production of oligosaccharides are currently available, but thechemo-enzymatic approach is very promising. Glycosynthases, mutated glycosidases that synthesize oligosaccharides in high yields, have been demonstrated to be an interesting alternative. However, examples of glycosynthases available so far are restricted to a limited number of glycosidases families and to only one retaining a-glycosynthase. We show here that newmutants of two a-L-fucosidases are efficient a-L-fucosynthases. The approach shown utilized b-L-fucopyranosyl azide as donor substrate leading to transglycosylation yields up to 91%. This is the first method exploiting a b-glycosyl azide donor for a-glycosynthases; its applicability to the glycosynthetic methodology in a wider perspective is presented. (literal)

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