http://www.cnr.it/ontology/cnr/individuo/prodotto/ID8987
An archaeal chaperonin-based reactor for renaturation of denatured proteins. (Articolo in rivista)
- Type
- Label
- An archaeal chaperonin-based reactor for renaturation of denatured proteins. (Articolo in rivista) (literal)
- Anno
- 2000-01-01T00:00:00+01:00 (literal)
- Alternative label
Cerchia L, Rossi M, Guagliardi A. (2000)
An archaeal chaperonin-based reactor for renaturation of denatured proteins.
in Extremophiles (Tokyo, Print)
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- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- Cerchia L, Rossi M, Guagliardi A. (literal)
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- ISI Web of Science (WOS) (literal)
- Titolo
- An archaeal chaperonin-based reactor for renaturation of denatured proteins. (literal)
- Abstract
- We describe an original chaperonin-based reactor that yields folded and active proteins from denatured materials. We used the 920-kDa chaperonin of the archaeon Sulfolobus solfataricus, which does not require any protein partner for its full activity and assists in vitro folding with low substrate specificity. The reactor consists of an ultrafiltration cell equipped with a membrane that retains the chaperonin in a functional state for folding in solution and permits the flowthrough of the folded substrates. By studying the ATP-dependent functional cycle of the chaperonin, we were able to use the reactor for repeated refolding processes. The scale-up of the reactor is made possible by the overproduction of chaperonin in Sulfolobus solfataricus cells that acquired thermotolerance upon appropriate heat shock.
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