Nanobeads-based assays. The case of gluten detection. (Articolo in rivista)

Type
Label
  • Nanobeads-based assays. The case of gluten detection. (Articolo in rivista) (literal)
Anno
  • 2008-01-01T00:00:00+01:00 (literal)
Alternative label
  • Venditti I, Frattodi I, Russo MV, Bellucci S, Crescenzo R, Iozzino L, Staiano M, Aurilia V, Varriale A, Rossi M, D'Auria S. (2008)
    Nanobeads-based assays. The case of gluten detection.
    in Journal of physics. Condensed matter (Online)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Venditti I, Frattodi I, Russo MV, Bellucci S, Crescenzo R, Iozzino L, Staiano M, Aurilia V, Varriale A, Rossi M, D'Auria S. (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 20 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#note
  • N. 474202 (3pp) (literal)
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Department of Chemistry, 'Sapienza' University of Rome, Piazzale Aldo Moro 5, Box 34 Rome 62, 00185 Rome, Italy INFN--Laboratori Nazionali di Frascati, Frascati, Italy Laboratory for Molecular Sensing, IBP-CNR, Via Pietro Castellino, 111 80131 Naples, Italy (literal)
Titolo
  • Nanobeads-based assays. The case of gluten detection. (literal)
Abstract
  • In order to verify if the use of nanobeads of poly[phenylacetylene-(co-acrylic acid)] (PPA/AA) in the ELISA test would affect the immune-activity of the antibodies (Ab) and/or the activity of the enzymes used to label the Ab anti-rabbit IGg, in this work we immobilized the horse liver peroxidase labelled Ab anti-rabbit IGg onto PPA/AA nanobeads. The gluten test was chosen as the model to demonstrate the usefulness of these nanobeads in immunoassays. The synthesis of PPA/AA nanobeads was performed by a modified emulsion polymerization. Self-assembly of nanospheres with mean diameter equal to 200 nm was achieved by casting aqueous suspensions. The materials were characterized by traditional spectroscopic techniques, while the size and dispersion of the particles were analysed by scanning electron microscopy (SEM) measurements. The obtained results show that the immobilization process of the Abs onto PPA/AA did not affect either the immune-response of the Abs or the functional activity of the peroxidase suggesting the usefulness of PPA/AA for the design of advanced nanobeads-based assays for the simultaneous screening of several analytes in complex media. (literal)
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