The peculiar structural features of kiwi fruit pectin methylesterase: Amino acid sequence, oligosaccharides structure, and modeling of the interaction with its natural proteinaceous inhibitor (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• The peculiar structural features of kiwi fruit pectin methylesterase: Amino acid sequence, oligosaccharides structure, and modeling of the interaction with its natural proteinaceous inhibitor (Articolo in rivista) (literal)

Anno

• 2008-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1002/prot.21681 (literal)

Alternative label

• Ciardiello M.A.; D'Avino R.; Amoresano A.; Tuppo L.; Carpentieri A.; Carratore V.; Tamburrini M.; Giovane A.; Pucci P.; Camardella L. (2008)
  The peculiar structural features of kiwi fruit pectin methylesterase: Amino acid sequence, oligosaccharides structure, and modeling of the interaction with its natural proteinaceous inhibitor
  in Proteins (Print)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Ciardiello M.A.; D'Avino R.; Amoresano A.; Tuppo L.; Carpentieri A.; Carratore V.; Tamburrini M.; Giovane A.; Pucci P.; Camardella L. (literal)

Pagina inizio

• 195 (literal)

Pagina fine

• 206 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 71 (literal)

Rivista

• Proteins (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 1 (literal)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Institute of Protein Biochemistry, C.N.R., 80131 Napoli, Italy Department of Organic Chemistry and Biochemistry, University Federico II of Napoli, 80126 Napoli, Italy Department of Biochemistry and Biophysics, Second University of Napoli, 80138 Napoli, Italy (literal)

Titolo

• The peculiar structural features of kiwi fruit pectin methylesterase: Amino acid sequence, oligosaccharides structure, and modeling of the interaction with its natural proteinaceous inhibitor (literal)

Abstract

• Pectin methylesterase (PME) from kiwi fruit (Actinidia deliciosa) is a glycoprotein, showing an apparent molecular mass of 50 kDa upon size exclusion chromatography and SDS-PAGE. The primary structure, elucidated by direct sequencing of the protein, comprises 321 amino acid residues providing a molecular mass of 35 kDa. The protein has an acetylated Thr residue at the amino terminus and five N-glycosylation consensus sequences, four of which are actually glycosylated. A careful investigation of the oligosaccharide structures demonstrated that PME glycans belong to complex type oligosaccharides essentially consisting of xylosylated polyfucosylated biantennary structures. Alignment with known mature plant PME sequences indicates that the postulated active site residues are conserved. Kiwi PME activity is inhibited following the interaction with the proteinaceous inhibitor PMEI, isolated from the same source. Gel-filtration experiments show that kiwi PME/PMEI complex is stable in a large pH range and dissociates only at pH 10.0. Modeling of the interaction with the inhibitor was performed by using the crystal structure of the complex between kiwi PMEI and tomato PME as a template. The model shows that the binding site is the same reported for tomato PME. However, additional salt link interactions are found to connect the external loops of kiwi PME to PMEI. This finding may explain the higher pH stability of the complex formed by the two kiwi proteins respect to that formed by PMEI and tomato PME. (literal)

Prodotto di

• DR. DE BERARDINIS: SV.P03.006 (SV.P14.003.001) (Modulo)
• Institute of protein biochemistry (IBP) (Istituto)
• DR. MANCO : Studi di idrolasi da fonti diverse e proteine vegetali. (SV.P14.010.001) (Modulo)

Autore CNR

• LAURA CAMARDELLA (Unità di personale interno)
• Lisa Tuppo (Unità di personale esterno)
• VITALE CARRATORE (Persona)
• ROSSANA D'AVINO (Persona)
• MARIA ANTONIETTA CIARDIELLO (Persona)
• MAURIZIO TAMBURRINI (Unità di personale interno)

Insieme di parole chiave

• Keywords of "The peculiar structural features of kiwi fruit pectin methylesterase: Amino acid sequence, oligosaccharides structure, and modeling of the interaction with its natural proteinaceous inhibitor" (Insieme di parole chiave)

Incoming links:

Autore CNR di

• MAURIZIO TAMBURRINI (Unità di personale interno)
• ROSSANA D'AVINO (Persona)
• LAURA CAMARDELLA (Unità di personale interno)
• VITALE CARRATORE (Persona)
• MARIA ANTONIETTA CIARDIELLO (Persona)
• Lisa Tuppo (Unità di personale esterno)

Prodotto

• Institute of protein biochemistry (IBP) (Istituto)
• DR. DE BERARDINIS: SV.P03.006 (SV.P14.003.001) (Modulo)
• DR. MANCO : Studi di idrolasi da fonti diverse e proteine vegetali. (SV.P14.010.001) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Proteins (Rivista)

Insieme di parole chiave di

• Keywords of "The peculiar structural features of kiwi fruit pectin methylesterase: Amino acid sequence, oligosaccharides structure, and modeling of the interaction with its natural proteinaceous inhibitor" (Insieme di parole chiave)