Gene cloning and expression in Escherichia coli of a bi-functional beta-D-xylosidase/alpha-L-arabinosidase from Sulfolobus solfataricus involved in xylan degradation (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• Gene cloning and expression in Escherichia coli of a bi-functional beta-D-xylosidase/alpha-L-arabinosidase from Sulfolobus solfataricus involved in xylan degradation (Articolo in rivista) (literal)

Anno

• 2007-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1007/s00792-006-0020-7 (literal)

Alternative label

• Morana A.; Paris O.; Maurelli L.; Rossi M.; Cannio R. (2007)
  Gene cloning and expression in Escherichia coli of a bi-functional beta-D-xylosidase/alpha-L-arabinosidase from Sulfolobus solfataricus involved in xylan degradation
  in Extremophiles (Tokyo, Print); Springer, tokio (Giappone)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Morana A.; Paris O.; Maurelli L.; Rossi M.; Cannio R. (literal)

Pagina inizio

• 123 (literal)

Pagina fine

• 132 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 11 (literal)

Rivista

• Extremophiles (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 1 (literal)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Institute of Protein Biochemistry - C.N.R., Via P. Castellino 111, 80131, Naples, Italy (literal)

Titolo

• Gene cloning and expression in Escherichia coli of a bi-functional beta-D-xylosidase/alpha-L-arabinosidase from Sulfolobus solfataricus involved in xylan degradation (literal)

Abstract

• An open reading frame encoding a putative bifunctional beta-D-xylosidase/alpha-L-arabinosidase (Sso3032) was identified on the genome sequence of Sulfolobus solfataricus P2, the predicted gene product showing high amino-acid sequence similarity to bacterial and eukaryal individual beta -D-xylosidases and alpha-L-arabinosidases as well as bi-functional enzymes such as the protein from Thermoanaerobacter ethanolicus and barley. The sequence was PCR amplified from genomic DNA of S. solfataricus P2 and heterologous gene expression obtained in Escherichia coli, under optimal conditions for overproduction. Specific assays performed at 75°C revealed the presence in the transformed E. coli cell extracts of this archaeal activity involved in sugar hydrolysis and specific for both substrates. The recombinant protein was purified by thermal precipitation of the host proteins and ethanol fractionation and other properties, such as high thermal activity and thermostability could be determined. The protein showed a homo-tetrameric structure with a subunit of molecular mass of 82.0 kDa which was in perfect agreement with that deduced from the cloned gene. Northern blot analysis of the xarS gene indicates that it is specifically induced by xylan and repressed by monosaccharides like D-glucose and L-arabinose. (literal)

Editore

• Springer (Editore)

Prodotto di

• Institute of protein biochemistry (IBP) (Istituto)
• PROF. M. ROSSI: Applicazioni innovative di enzimi e biotrasformazioni (SV.P14.002.001) (Modulo)

Autore CNR

• ALESSANDRA MORANA (Persona)
• RAFFAELE CANNIO (Persona)
• LUISA MAURELLI (Persona)
• Mosè Rossi (Unità di personale esterno)

Insieme di parole chiave

• Keywords of "Gene cloning and expression in Escherichia coli of a bi-functional beta-D-xylosidase/alpha-L-arabinosidase from Sulfolobus solfataricus involved in xylan degradation" (Insieme di parole chiave)

Incoming links:

Autore CNR di

• RAFFAELE CANNIO (Persona)
• LUISA MAURELLI (Persona)
• Mosè Rossi (Unità di personale esterno)
• ALESSANDRA MORANA (Persona)

Prodotto

• Institute of protein biochemistry (IBP) (Istituto)
• PROF. M. ROSSI: Applicazioni innovative di enzimi e biotrasformazioni (SV.P14.002.001) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Extremophiles (Rivista)

Editore di

• Springer (Editore)

Insieme di parole chiave di

• Keywords of "Gene cloning and expression in Escherichia coli of a bi-functional beta-D-xylosidase/alpha-L-arabinosidase from Sulfolobus solfataricus involved in xylan degradation" (Insieme di parole chiave)