A new kumamolisin-like protease from Alicyclobacillus acidocaldarius: an enzyme active under extreme acidic conditions. (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• A new kumamolisin-like protease from Alicyclobacillus acidocaldarius: an enzyme active under extreme acidic conditions. (Articolo in rivista) (literal)

Anno

• 2006-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1080/10242420600792094 (literal)

Alternative label

• Catara G.; Fiume I.; Iuliano F.; Maria G.; Ruggiero G.; Palmieri G.; Capasso A.; Rossi M. (2006)
  A new kumamolisin-like protease from Alicyclobacillus acidocaldarius: an enzyme active under extreme acidic conditions.
  in Biocatalysis and biotransformation (Print)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Catara G.; Fiume I.; Iuliano F.; Maria G.; Ruggiero G.; Palmieri G.; Capasso A.; Rossi M. (literal)

Pagina inizio

• 358 (literal)

Pagina fine

• 370 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#altreInformazioni

• Prodotto digitalizzato (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 24 (literal)

Rivista

• Biocatalysis and biotransformation (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 5 (literal)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Istituto di Biochimica delle Proteine CNR (literal)

Titolo

• A new kumamolisin-like protease from Alicyclobacillus acidocaldarius: an enzyme active under extreme acidic conditions. (literal)

Abstract

• A new serine-carboxyl proteinase, called kumamolisin-ac, was purified from the thermoacidophilic bacterium Alicyclobacillus acidocaldarius. The enzyme is a monomeric protein of 45 kDa, active over a wide temperature range (5.0–70°C) and extremely acidic pHs (1.0–4.0), showing maximal proteolytic activity at pH 2.0 and 60°C. Interestingly, kumamolisin-ac displayed a significant proteolytic activity even at 5°C, thus suggesting a sort of cold-adaptation for this enzyme. The protease was remarkably stable at high temperatures (t1/2 at 80°C, 10h, pH 2.0) and over a broad range of pH (2.0–7.0). Substrate analysis indicated that kumamolisin-ac was active on a variety of macromolecular substrates, such as haemoglobin, hide powder azure, and azocoll. In particular, a high specific activity was detected towards collagen. The corresponding gene was cloned, expressed and the recombinant protease, was found to be homologous to proteases of the ‘S53’ family. From the high identity with kumamolisin and kumamolisin-As, known as collagenolytic proteases, kumamolisin-ac can be considered as the third collagenolytic affiliate within the ‘S53’ family. Cleavage specificity investigation of kumamolisin-ac revealed a unique primary cleavage site in bovine insulin B-chain, whereas a broad specificity was detected using bovine alpha-globin as substrate. Thus, kumamolisin-ac could represent an attractive candidate for industrial-scale biopeptide production under thermoacidophilic conditions. (literal)

Prodotto di

• Institute of protein biochemistry (IBP) (Istituto)
• PROF. M. ROSSI: Applicazioni innovative di enzimi e biotrasformazioni (SV.P14.002.001) (Modulo)

Autore CNR

• GIANNA PALMIERI (Persona)
• ANTONIO CAPASSO (Unità di personale interno)
• IMMACOLATA FIUME (Persona)
• GIUSEPPE RUGGIERO (Persona)
• GIULIANA CATARA (Unità di personale esterno)
• Mosè Rossi (Unità di personale esterno)

Insieme di parole chiave

• Parole chiave di "A new kumamolisin-like protease from Alicyclobacillus acidocaldarius: an enzyme active under extreme acidic conditions." (Insieme di parole chiave)

Incoming links:

Autore CNR di

• GIANNA PALMIERI (Persona)
• GIUSEPPE RUGGIERO (Persona)
• Mosè Rossi (Unità di personale esterno)
• IMMACOLATA FIUME (Persona)
• ANTONIO CAPASSO (Unità di personale interno)
• GIULIANA CATARA (Unità di personale esterno)

Prodotto

• Institute of protein biochemistry (IBP) (Istituto)
• PROF. M. ROSSI: Applicazioni innovative di enzimi e biotrasformazioni (SV.P14.002.001) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Biocatalysis and biotransformation (Rivista)

Insieme di parole chiave di

• Parole chiave di "A new kumamolisin-like protease from Alicyclobacillus acidocaldarius: an enzyme active under extreme acidic conditions." (Insieme di parole chiave)