Identification and autonomous replication capability of a chromosomal replication origin from the archaeon Sulfolobus solfataricus. (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Identification and autonomous replication capability of a chromosomal replication origin from the archaeon Sulfolobus solfataricus. (Articolo in rivista) (literal)

Anno

• 2004-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1007/s00792-004-0399-y (literal)

Alternative label

• Contursi P.; Pisani F.M.; Grigoriev A.; Cannio R.; Bartolucci S.; Rossi M. (2004)
  Identification and autonomous replication capability of a chromosomal replication origin from the archaeon Sulfolobus solfataricus.
  in Extremophiles (Tokyo, Print)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Contursi P.; Pisani F.M.; Grigoriev A.; Cannio R.; Bartolucci S.; Rossi M. (literal)

Pagina inizio

• 385 (literal)

Pagina fine

• 391 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 8 (literal)

Rivista

• Extremophiles (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 5 (literal)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• CNR IMM (literal)

Titolo

• Identification and autonomous replication capability of a chromosomal replication origin from the archaeon Sulfolobus solfataricus. (literal)

Abstract

• Here, we describe the identification of a chromosomal DNA replication origin (oriC) from the hyperthermophilic archaeon Sulfolobus solfataricus (subdomain of Crenarchaeota). By means of a cumulative GC-skew analysis of the Sulfolobus genome sequence, a candidate oriC was mapped within a 1.12-kb region located between the two divergently transcribed MCM- and cdc6-like genes. We demonstrated that plasmids containing the Sulfolobus oriC sequence and a hygromycin-resistance selectable marker were maintained in an episomal state in transformed S. solfataricus cells under selective pressure. The proposed location of the origin was confirmed by 2-D gel electrophoresis experiments. This is the first report on the functional cloning of a chromosomal oriC from an archaeon and represents an important step toward the reconstitution of an archaeal in vitro DNA replication system. (literal)

Prodotto di

• Institute of protein biochemistry (IBP) (Istituto)

Autore CNR

• RAFFAELE CANNIO (Persona)
• FRANCESCA MARIA PISANI (Persona)
• Mosè Rossi (Unità di personale esterno)

Incoming links:

Autore CNR di

• RAFFAELE CANNIO (Persona)
• Mosè Rossi (Unità di personale esterno)
• FRANCESCA MARIA PISANI (Persona)

Prodotto

• Institute of protein biochemistry (IBP) (Istituto)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Extremophiles (Rivista)