Isolation and Characterization of Genes for the Synthesis of Chlorogenic Acid in Artichoke (Comunicazione a convegno)

Type
Label
  • Isolation and Characterization of Genes for the Synthesis of Chlorogenic Acid in Artichoke (Comunicazione a convegno) (literal)
Anno
  • 2009-01-01T00:00:00+01:00 (literal)
Alternative label
  • Sonnante G., D'’Amore R., Blanco E., Luo J., Pignone D., Martin C. (2009)
    Isolation and Characterization of Genes for the Synthesis of Chlorogenic Acid in Artichoke
    in 7th International Symposium on Artichoke, Saint Pol de Léon, France, 17-19 giugno 2009
    (literal)
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  • Sonnante G., D'’Amore R., Blanco E., Luo J., Pignone D., Martin C. (literal)
Pagina inizio
  • 27 (literal)
Pagina fine
  • 27 (literal)
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  • PRESENTAZIONE ORALE (literal)
Note
  • Abstract (literal)
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  • CNR-IGV Bari; John Innes Centre, Norwich, UK (literal)
Titolo
  • Isolation and Characterization of Genes for the Synthesis of Chlorogenic Acid in Artichoke (literal)
Abstract
  • It is well known that artichoke possesses a number of beneficial properties, such as antioxidant activity, hepatoprotective, coleretic, and hypocolesterol properties, mainly attributable to polyphenols, a class of compounds with beneficial effects on human health. These are secondary metabolites including hydroxycinnamates, particularly caffeic acid, chlorogenic acid (CGA), di-caffeoylquinic acids, and flavonoids (e.g. luteolin). Chlorogenic acid, in particular, is a potent antioxidant very abundant in some vegetables, included artichoke. There are three proposed routes for the synthesis of CGA in plants, and the enzyme hydroxycynnamoyl-quinate transferase (HQT), belonging to the BAHD family is reported to be involved in the last step of the biochemical pathway in some plant species. In artichoke the synthesis of CGA has not been elucidated yet. In the present contribution, we report on the isolation and characterization of two artichoke full-length hqt and hqt2 cDNA sequences. Genomic organization of the genes revealed that they contain two exons and one intron. The obtained sequences showed a high level of similarity to homologous genes from other plant species, particularly tobacco and tomato. The transcripts of the two artichoke genes were heterologously expressed and biochemical assays were performed, in order to assess specificity of the enzymes for various substrates. Results indicate that quinate is preferred over shikimate, confirming that the two artichoke HQT enzymes possess an activity similar to other HQTs. Real time PCR in different plant parts and developmental stages revealed that the two genes are differentially expressed in artichoke plants. A better understanding of how the artichoke genes involved in the pathway leading to CGA and di-caffeoylquinic acids production interact is important to develop agronomic, genetic, or biotechnological tools for higher production of biologically active compounds in artichoke. (literal)
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