Heterologous expression of an artichoke cytochrome P450 gene affects flavonoid biosynthesis in transgenic tobacco plants (Abstract/Poster in atti di convegno)

Type

• Abstract/Poster in atti di convegno (Classe)
• Prodotto della ricerca (Classe)

Label

• Heterologous expression of an artichoke cytochrome P450 gene affects flavonoid biosynthesis in transgenic tobacco plants (Abstract/Poster in atti di convegno) (literal)

Anno

• 2008-01-01T00:00:00+01:00 (literal)

Alternative label

• De Palma M, Fratianni F., Nazzaro F., Tucci M (2008)
  Heterologous expression of an artichoke cytochrome P450 gene affects flavonoid biosynthesis in transgenic tobacco plants
  in Atti del LII Convegno SIGA, Padova, 14-17 Settembre 2008
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• De Palma M, Fratianni F., Nazzaro F., Tucci M (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#altreInformazioni

• Abstract B16 (literal)

Note

• Poster (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• CNR-IGV, Portici; ISA Avellino (literal)

Titolo

• Heterologous expression of an artichoke cytochrome P450 gene affects flavonoid biosynthesis in transgenic tobacco plants (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#isbn

• 978-88-900622-8-5 (literal)

Abstract

• Globe artichoke (Cynara cardunculus var. scolymus L.) is a typical component of the Mediterranean diet, and has interesting applications in pharmacology due to its beneficial properties. These properties have been attributed to the phenolic compounds, among which the flavonoid luteolin has been found to be a powerful antioxidant and inhibitor of cholesterol biosynthesis. Flavonoids are plant secondary metabolites with essential physiological function, whose central biosynthetic pathway is highly conserved in plants. The luteolin biosynthetic pathway has been elucidated in Arabidopsis and other species and many structural genes have been isolated and characterized (i.e. flavonoid 3'- hydroxylase, F3'H and flavone synthase II, FNSII). However, in artichoke this pathway is poorly characterized and no information are available yet about the key genes that are involved in luteolin synthesis. In this study, we isolated the two artichoke cytochrome P450-encoding genes F3'H and FNSII by homology-based PCR approach and performed transcription analyses in different tissues and organs of several artichoke local and commercial varieties in order to assess differential expression patterns of the two genes. To demonstrate whether the putative F3'H artichoke gene has enzymatic activity in vivo and is involved in luteolin biosynthesis in artichoke, transgenic tobacco plants overespressing F3'H were obtained. In some tobacco primary transgenics, changes in flower colour intensity were observed indicating that this gene is involved in flower colour modification in tobacco. Moreover, the main phenolic compounds were analyzed by HPLC in leaves and flowers at different development stages, detecting several significant changes. Further research will be focused on the full characterization of the activities of the artichoke F3'H and FNSII-encoded enzymes. (literal)

Prodotto di

• Istituto di Bioscienze e Biorisorse (IBBR) (Istituto)
• Ottenimento di nuovi prodotti per la salute, l'alimentazione e l'industria mediante interventi genetici e biotecnologici nelle piante (AG.P01.006.001) (Modulo)

Autore CNR

• MARINA TUCCI (Unità di personale interno)
• FILOMENA NAZZARO (Persona)
• MONICA DE PALMA (Persona)
• FLORINDA FRATIANNI (Unità di personale interno)

Incoming links:

Autore CNR di

• FILOMENA NAZZARO (Persona)
• MONICA DE PALMA (Persona)
• FLORINDA FRATIANNI (Unità di personale interno)
• MARINA TUCCI (Unità di personale interno)

Prodotto

• Istituto di Bioscienze e Biorisorse (IBBR) (Istituto)
• Ottenimento di nuovi prodotti per la salute, l'alimentazione e l'industria mediante interventi genetici e biotecnologici nelle piante (AG.P01.006.001) (Modulo)