Genetic diversity of Italian lentil landraces using microsatellite markers (Abstract/Poster in atti di convegno)

Type
Label
  • Genetic diversity of Italian lentil landraces using microsatellite markers (Abstract/Poster in atti di convegno) (literal)
Anno
  • 2007-01-01T00:00:00+01:00 (literal)
Alternative label
  • Sonnante G., Santantonio M., Sonnante G., Lioi L. (2007)
    Genetic diversity of Italian lentil landraces using microsatellite markers
    in Atti del “51° Convegno annuale SIGA”, Riva del Garda (TN), 23-26 Settembre 2007
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Sonnante G., Santantonio M., Sonnante G., Lioi L. (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#note
  • ISBN 978-88-900622-7-8, A.26. (literal)
Note
  • Abstract (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • IGV-CNR (literal)
Titolo
  • Genetic diversity of Italian lentil landraces using microsatellite markers (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#isbn
  • 978-88-900622-7-8 (literal)
Abstract
  • Lentil cultivation in Italy is mainly based on landraces, local varieties empirically selected by farmers over time and well adapted to the agro-environment in which they have been grown for decades. Most landraces survive on farm, in marginal areas and exposed to a strong risk of genetic erosion and/or extinction. In order to assess genetic diversity and relationships, nine local varieties, two lines and two cultivars of lentil were analysed using microsatellite (SSR) markers. Ten individuals for each landrace and line were used. Plants were grown in a greenhouse and DNA was extracted from young leaves. Sixteen primer pairs, reported to amplify microsatellite regions in lentil, were selected from the published literature. One primer for each pair was fluorescently labelled, so that amplified fragments could be visualized on an automated sequencer. All the used primer pairs produced an amplification product of the expected length. A total of 170 alleles were scored, ranging from 1 to 22 alleles per locus. The diversity parameters were calculated using PopGene software, and resulted to be very low for the selected lines and the cultivars. On the other hand, the lentil landraces analysed in the present study showed quite high values for all the genetic diversity parameters, especially when compared to other legumes cultivated in Italy, such as common bean, or chickpea. These results indicate that the examined landraces retain a high level of genetic diversity among single genotypes of the same landrace. The highest levels of genetic diversity were observed for Castelluccio, Colliano, and Villalba landraces. Microsatellite allele frequencies were used to calculate Nei's genetic distances, and a UPGMA dendrogram was constructed. As a general rule, lentil landraces were grouped according to their geographical origin. For the ability of SSRs to detect allelic differences between individuals, these sequences have currently become the markers of choice for assessing genetic variation within and among landraces. (literal)
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