http://www.cnr.it/ontology/cnr/individuo/prodotto/ID82369
Genetic diversity of Italian lentil landraces using microsatellite markers (Abstract/Poster in atti di convegno)
- Type
- Label
- Genetic diversity of Italian lentil landraces using microsatellite markers (Abstract/Poster in atti di convegno) (literal)
- Anno
- 2007-01-01T00:00:00+01:00 (literal)
- Alternative label
Sonnante G., Santantonio M., Sonnante G., Lioi L. (2007)
Genetic diversity of Italian lentil landraces using microsatellite markers
in Atti del 51° Convegno annuale SIGA, Riva del Garda (TN), 23-26 Settembre 2007
(literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- Sonnante G., Santantonio M., Sonnante G., Lioi L. (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#note
- ISBN 978-88-900622-7-8, A.26. (literal)
- Note
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- Titolo
- Genetic diversity of Italian lentil landraces using microsatellite markers (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#isbn
- 978-88-900622-7-8 (literal)
- Abstract
- Lentil cultivation in Italy is mainly based on landraces, local varieties empirically selected by
farmers over time and well adapted to the agro-environment in which they have been grown for
decades. Most landraces survive on farm, in marginal areas and exposed to a strong risk of genetic
erosion and/or extinction. In order to assess genetic diversity and relationships, nine local varieties,
two lines and two cultivars of lentil were analysed using microsatellite (SSR) markers. Ten
individuals for each landrace and line were used. Plants were grown in a greenhouse and DNA was
extracted from young leaves. Sixteen primer pairs, reported to amplify microsatellite regions in
lentil, were selected from the published literature. One primer for each pair was fluorescently
labelled, so that amplified fragments could be visualized on an automated sequencer. All the used
primer pairs produced an amplification product of the expected length. A total of 170 alleles were
scored, ranging from 1 to 22 alleles per locus. The diversity parameters were calculated using
PopGene software, and resulted to be very low for the selected lines and the cultivars. On the other
hand, the lentil landraces analysed in the present study showed quite high values for all the genetic
diversity parameters, especially when compared to other legumes cultivated in Italy, such as
common bean, or chickpea. These results indicate that the examined landraces retain a high level of
genetic diversity among single genotypes of the same landrace. The highest levels of genetic
diversity were observed for Castelluccio, Colliano, and Villalba landraces. Microsatellite allele
frequencies were used to calculate Nei's genetic distances, and a UPGMA dendrogram was
constructed. As a general rule, lentil landraces were grouped according to their geographical origin.
For the ability of SSRs to detect allelic differences between individuals, these sequences have
currently become the markers of choice for assessing genetic variation within and among landraces. (literal)
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