Modified multi-walled carbon nanotubes potentially suitable for intracellular pH measurements (Contributo in atti di convegno)

Type
Label
  • Modified multi-walled carbon nanotubes potentially suitable for intracellular pH measurements (Contributo in atti di convegno) (literal)
Anno
  • 2010-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1117/12.843556 (literal)
Alternative label
  • G. Ghini; G.L. Puleo; C. Trono; A. Giannetti; L. Luconi; C. Bianchini; G. Giambastiani; F. Baldini (2010)
    Modified multi-walled carbon nanotubes potentially suitable for intracellular pH measurements
    in Proc.SPIE 7574, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications VII, San Francisco
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • G. Ghini; G.L. Puleo; C. Trono; A. Giannetti; L. Luconi; C. Bianchini; G. Giambastiani; F. Baldini (literal)
Pagina inizio
  • 75740C1 (literal)
Pagina fine
  • 75740C8 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#titoloVolume
  • Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications VII (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#volumeInCollana
  • 7574 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali
  • 8 (literal)
Note
  • Scopu (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Istuituto di Fisica Applicata -IFAC Istituto di Chimica dei Composti Organometallici - ICCOM (literal)
Titolo
  • Modified multi-walled carbon nanotubes potentially suitable for intracellular pH measurements (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#isbn
  • 9780819479709 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autoriVolume
  • Alexander N. Cartwright; Dan V. Nicolau (literal)
Abstract
  • Carboxylic acid functionalized multi-walled carbon nanotubes (COOH-MWCNTs) have been studied as macromolecular carriers for pH indicators to be used inside cells. The activation of carboxylic groups with thionyl chloride (SOCl2) followed by the reaction with a family of fluorescein ethylen glycol derivatives led to dyes covalently anchored to the MWCNT surface. Such a functionalization was found to preserve wholly the fluorescence properties of the dye ultimately providing higher water solubility to the modified macromolecular systems. Moreover, the use of a polyether spacer between the dye and the MWCNT surface preserved from undesired florescence quenching effects. The pH dependence of the modified nanotubes was investigated interrogating a solution of MWCNTs, the pH of which was adjusted in the range 4-9 pH units by adding drops of hydrochloric acid and sodium hydroxide. Light from LED was suitably filtered at 480 nm with a high pass-band filter and coupled to an optical fiber which illuminates the solution containing the fluorescein-functionalised MWCNTs. An optical fiber, at 90° with respect to the LED illumination, is connected with a Hamamtsu spectrum analyzer for the recording of the fluorescence spectra. The modified MWCNTs exhibited linear pH dependence in the range between 6 and 8 pH units with a sensitivity less than 0.1 pH units. (literal)
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