Healing process study of laser-welded corneal tissue by Multispectral Imaging Autofluorescence Microscopy (MIAM) (Contributo in atti di convegno)

Type
Label
  • Healing process study of laser-welded corneal tissue by Multispectral Imaging Autofluorescence Microscopy (MIAM) (Contributo in atti di convegno) (literal)
Anno
  • 2006-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1117/12.647432 (literal)
Alternative label
  • R.Pini, V.Basile, S.Ambrosini, G.Vannelli, F. Rossi, L.Menabuoni, R.Pratesi, M.Monici (2006)
    Healing process study of laser-welded corneal tissue by Multispectral Imaging Autofluorescence Microscopy (MIAM)
    in Ophthalmic Technologies XVI, SPIE Biomedical Optics 2006, San Josè, CA, USA, 21 January 2006 through 24 January 2006
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • R.Pini, V.Basile, S.Ambrosini, G.Vannelli, F. Rossi, L.Menabuoni, R.Pratesi, M.Monici (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#titoloVolume
  • Ophthalmic Technologies XVI (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#volumeInCollana
  • 6138 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#note
  • Pubblicato in Ophthalmic Technologies XVI, SPIE Vol. 6138, pp. 404-409, Bellingham, WA, USA, ISBN: 0-8194-6181-4 (2006) (literal)
Note
  • ISI Web of Science (WOS) (literal)
  • Google Scholar (literal)
  • Scopu (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • IFAC CNR Firenze Dipartimento di Fisiopatologia Clinica-Sez.Fisica Medica, Università degli Studi di Firenze Dipartimento di Anatomia, Istologia e Medicina Legale, Università degli Studi di Firenze Dipartimento di Fisica, Università di Firenze CEO-Centro di Eccellenza Optronica, Firenze (literal)
Titolo
  • Healing process study of laser-welded corneal tissue by Multispectral Imaging Autofluorescence Microscopy (MIAM) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#isbn
  • 0-8194-6181-4 (literal)
Abstract
  • Laser welding of corneal tissue is an alternative technique to conventional suturing procedures in ophthalmic surgery. The welding effect is achieved after staining the wound with a chromophore (Indocyanine Green, shortly: ICG) and then irradiating it with a low power diode laser. We present a study on the healing process of comeal wounds using Multispectral Imaging Autofluorescence Microscopy (MIAM). This technique is based on the characterization of fluorescence arising from tissue components (autofluorescence): it is particularly useful in studying corneal tissue, because it is mainly composed of type I collagen, one of the most important endogenous fluorophores. Laser welding tests of the cornea were carried out on rabbits in which full thickness corneal cuts of about 5 mm were sutured using a diode laser emitting at 810 nm, with a power of 80 mW. Bioptic sections of rabbit corneas were examined in a follow up study of 90 days after surgery, and the results were complementary to histological analysis performed in previous studies. Autofluorescence images showed a faster healing process and a better reorganization of the architecture of stromal fibers, in comparison with conventional suturing procedures. MIAM technique can represent a new tool to study the morphology of corneal tissue, offering some real advantages with respect to standard histological analysis. In fact, it does not require any chemical manipulation of the samples, providing information on the biological structure by directly monitoring distribution and emission intensity of endogenous fluorophores. (literal)
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