http://www.cnr.it/ontology/cnr/individuo/prodotto/ID79022
Unraveling the binding mode of the neutralizing neuroantibody aD11 to NGF (Contributo in atti di convegno)
- Type
- Label
- Unraveling the binding mode of the neutralizing neuroantibody aD11 to NGF (Contributo in atti di convegno) (literal)
- Anno
- 2005-01-01T00:00:00+01:00 (literal)
- Alternative label
S. Covaceuszach, A. Cassetta, A. Cattaneo, D. Lamba (2005)
Unraveling the binding mode of the neutralizing neuroantibody aD11 to NGF
in XX Congress of the International Union of Crystallography, Florence
(literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- S. Covaceuszach, A. Cassetta, A. Cattaneo, D. Lamba (literal)
- Pagina inizio
- Pagina fine
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
- Rivista
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#note
- XX Congress of the International Union of Crystallography:
Congress and General Assembly
23-31 August 2005
Acta Cryst. (2005) A61, c234
(literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- S. Covaceuszach -SISSA, Trieste; LLG-Roma
A. Cassetta -IC-CNR, Trieste
A. Cattaneo -SISSA, Trieste; LLG-Roma; EBRI-Roma
D. Lamba -IC-CNR, Trieste (literal)
- Titolo
- Unraveling the binding mode of the neutralizing neuroantibody aD11 to NGF (literal)
- Abstract
- The monoclonal neuroantibody ?D11 is a potent antagonist that
prevents the binding of the neurotrophin NGF (nerve growth factor) to
its receptors, TrkA and p75, in a variety of systems, most notably in
two in vivo systems linked to crucial pathological states (i.e.
Alzheimer disease and AIDS). To elucidate the mechanism of
neutralization, structural and functional studies were performed.
The potential therapeutical interest of the antibody was confirmed,
demonstrating that it binds both mouse and human NGF with similar
affinity. Its epitope was mapped by Sandwich Elisa assay with a pool
of mutants. Its Fab fragment was crystallized [1] and the structure was
solved at 1.80Å resolution. This structure, that may assist in the
humanization without loss in affinity, was docked to NGF on the basis
of epitope mapping results. (literal)
- Prodotto di
- Autore CNR
Incoming links:
- Prodotto
- Autore CNR di
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi