The MALDI TOF/TOF Mass Spectrometer as a Chemical Lab for the structural characterization of lipooligosaccharides (Contributo in atti di convegno)

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  • The MALDI TOF/TOF Mass Spectrometer as a Chemical Lab for the structural characterization of lipooligosaccharides (Contributo in atti di convegno) (literal)
Anno
  • 2011-01-01T00:00:00+01:00 (literal)
Alternative label
  • L.Sturiale, A.Palmigiano, A.Silipo, R.Lanzetta, M.Parrilli, A.Molinaro, D.Garozzo (2011)
    The MALDI TOF/TOF Mass Spectrometer as a Chemical Lab for the structural characterization of lipooligosaccharides
    in 16th European Carbohydrate Symposium, Sorrento - Naples, Italy
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • L.Sturiale, A.Palmigiano, A.Silipo, R.Lanzetta, M.Parrilli, A.Molinaro, D.Garozzo (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#altreInformazioni
  • 45 (2011). Conference Proceeding –Invited lecture (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#note
  • July 3-7. (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#descrizioneSinteticaDelProdotto
  • Abstract: Lipooligosaccharide (LOS) is a powerful Gram negative glycolipid involved in immune system elusion and invasion of host animal and vegetal cells. Its structural elucidation is pivotal to understand at molecular levels mechanisms of infections. Due to its amphiphilic nature, it has resisted structural matrix assisted laser desorption/ionization (MALDI) analysis. Our approach has now resolved this important issue, permitting us to obtain high resolution MALDI mass spectra that are rich of information. In fact, they show both LOS quasimolecular ions, and also well defined fragments due to a in-source decay which yields B-type ions corresponding to core oligosaccharide(s), and Y-type ions relating to lipid A unit(s). Moreover, MALDI tandem Time of Flight (TOF/TOF) mass spectrometry (MS) of lipid A allows its complete structural elucidation straight from the purified intact LOS, without any chemical manipulation. These findings constitute a significant improvement in the analysis of such important biomolecule by MS. (literal)
Titolo
  • The MALDI TOF/TOF Mass Spectrometer as a Chemical Lab for the structural characterization of lipooligosaccharides (literal)
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