Assessment of Escherichia coli viability in coastal Sicilian waters by fluorescent antibody and beta-glucuronidase activity methods. (Contributo in atti di convegno)

Type

• Contributo in atti di convegno (Classe)
• Prodotto della ricerca (Classe)

Label

• Assessment of Escherichia coli viability in coastal Sicilian waters by fluorescent antibody and beta-glucuronidase activity methods. (Contributo in atti di convegno) (literal)

Anno

• 2006-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.2495/CENV060061 (literal)

Alternative label

• Caruso G., Zappalà G., Caruso R., Crisafi E (2006)
  Assessment of Escherichia coli viability in coastal Sicilian waters by fluorescent antibody and beta-glucuronidase activity methods.
  in 6. International Conference on Environmental Problems in Coastal Regions, Rhodes (Greece), 2006
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Caruso G., Zappalà G., Caruso R., Crisafi E (literal)

Pagina inizio

• 57 (literal)

Pagina fine

• 66 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#altreInformazioni

• SCIMAGO SJR 2011: 0.131, SJR 2009: 0.108 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#titoloVolume

• Environmental Problems in Coastal Regions VI: Including Oil Spill Studies (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#volumeInCollana

• 88 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#note

• In: Brebbia C.A. (ed.), Environmental Problems in Coastal Regions VI,Proceedings, pp. 57-66, WIT Press, Southampton (UK). (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali

• 10 (literal)

Note

• ISI Web of Science (WOS) (literal)
• Google Scholar (literal)
• Scopu (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• CNR-IAMC Messina A.O.U. \"G. Martino\" Messina (literal)

Titolo

• Assessment of Escherichia coli viability in coastal Sicilian waters by fluorescent antibody and beta-glucuronidase activity methods. (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#isbn

• 978-1-84564-167-2 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#curatoriVolume

• Brebbia C.A. (literal)

Abstract

• Monitoring the viability of pathogens in bathing waters is crucial for public health preservation. The fluorescent antibody (FA)-viability method, using antibodies for Escherichia coli combined with CTC (5-cyano-2, 3 ditolyl tetrazolium chloride, marker of active respiring cells) or PI (propidium iodide, marker of membrane-damaged cells), and the B-glucuronidase activity test (by 4- methylumbelliferyl-b-d-glucuronide, MUG) offer interesting perspectives for detecting this bacterial indicator of faecal pollution. The viability of E. coli cells in Sicilian seawaters was evaluated on a seasonal scale (n = 89 samples) by FA and MUG compared to the standard culture method (by m-FC agar). The total microscopic counts obtained exceeded the culturable counts (m-FC) by one order of magnitude; actively respiring (CTC+) cells accounted, on average, for 63% of the total E. coli population. B-glucuronidase activity rates ranged from 0.00004 to 726.88 nmol MUF 100 ml-1h-1, and were particularly enhanced during autumn and spring. The percentage of membrane-damaged cells increased in summer and in winter, probably due to adverse environmental conditions. In winter, high numbers of culturable cells suggested continental run-off to be an important source of pathogens; bacterial cells, however, were not actively respiring, but were membrane-damaged or with low metabolism, as indicated by the increase in PI counts and the decrease in MUG rates, respectively. In spring, increased numbers of CTC (+) cells and B-glucuronidase rates suggested the recovery of some metabolic functions. Both methods used provided evidence that some viability properties were retained by E. coli cells after their discharge into seawater; therefore their combination could be proposed as a useful and rapid tool for environmental monitoring. (literal)

Editore

• Wit Press (Editore)

Prodotto di

• Qualità del Mare e delle Acque Costiere: (TA.P03.003.001) (Modulo)
• Approccio integrato per un'acquacoltura sostenibile: aspetti ambientali, qualità, benessere e applicazioni di nuove tecnologie (TA.P04.019.001) (Modulo)
• Institute for coastal marine environment (IAMC) (Istituto)

Autore CNR

• GIUSEPPE ZAPPALA' (Persona)
• GABRIELLA CARUSO (Persona)
• ERMANNO CRISAFI (Unità di personale interno)

Insieme di parole chiave

• Parole chiave di "Assessment of Escherichia coli viability in coastal Sicilian waters by fluorescent antibody and beta-glucuronidase activity methods." (Insieme di parole chiave)

Incoming links:

Prodotto

• Approccio integrato per un'acquacoltura sostenibile: aspetti ambientali, qualità, benessere e applicazioni di nuove tecnologie (TA.P04.019.001) (Modulo)
• Institute for coastal marine environment (IAMC) (Istituto)
• Qualità del Mare e delle Acque Costiere: (TA.P03.003.001) (Modulo)

Autore CNR di

• GIUSEPPE ZAPPALA' (Persona)
• ERMANNO CRISAFI (Unità di personale interno)
• GABRIELLA CARUSO (Persona)

Editore di

• Wit Press (Editore)

Insieme di parole chiave di

• Parole chiave di "Assessment of Escherichia coli viability in coastal Sicilian waters by fluorescent antibody and beta-glucuronidase activity methods." (Insieme di parole chiave)