Molecular markers for cultivar characterisation in Olea europaea (Articolo in rivista)

Type
Label
  • Molecular markers for cultivar characterisation in Olea europaea (Articolo in rivista) (literal)
Anno
  • 2003-01-01T00:00:00+01:00 (literal)
Alternative label
  • Bernardi R.1, Manzo M.1, Durante M.1, Petruccelli R.2, Bartolini G.2 (2003)
    Molecular markers for cultivar characterisation in Olea europaea
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Bernardi R.1, Manzo M.1, Durante M.1, Petruccelli R.2, Bartolini G.2 (literal)
Pagina inizio
  • 97 (literal)
Pagina fine
  • 100 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#altreInformazioni
  • Marcatori molecolari utilizzabili per la identificazione varietale e certificazione. (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 586 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#descrizioneSinteticaDelProdotto
  • The olive cultivars Arancino, Carboncella, Corniolo, Moraiolo and Tondello have been characterised by different molecular methodologies. Analyses for olive cultivar identification were carried out through PCR amplification and cloning of different DNA sequences. Among these, two concerned the analysis of the ribosomal internal spacers (ITSs) and the products of random amplified polymorphic DNA. The nuclear rDNA region, comprising the first internal transcribed spacer (ITS1), the 5.8S rRNA gene and the second internal transcribed spacer (ITS2), was amplified by the polymerase chain reaction (PCR) by using two primers, respectively complementary to the 18S and 25S rDNA near the ITS1 and ITS2 borders. The PCR products amplified from the samples appeared as a single band of approximately 700 bp in size and they were cloned using the pCR 2.1 Vector. The ITS1 (about 250 bp), ITS2 (about 200 bp) and 5.8S rRNA gene were completely sequenced. Sequence analyses were used to evaluate the genetic diversity among cultivars. The DNA aligned sequences were compared with published sequences of other Oleaceae genera available in GenBank. Moreover, the multiple alignments were used to reconstruct the genetic distances of the Olea cultivars. In the second approach, RAPD analysis was carried out by using 40 different primers after purification of highly polymerised DNA. The results obtained through the two above mentioned methods evidenced differences in DNA components among cultivars. (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • 1 Uni Pisa 2 CNR (literal)
Titolo
  • Molecular markers for cultivar characterisation in Olea europaea (literal)
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