http://www.cnr.it/ontology/cnr/individuo/prodotto/ID67872
Fluorescence In Situ Hybridization in soil and water ecosystems: an useful method for studying the effect of xenobiotics on bacterial community structure (Articolo in rivista)
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- Label
- Fluorescence In Situ Hybridization in soil and water ecosystems: an useful method for studying the effect of xenobiotics on bacterial community structure (Articolo in rivista) (literal)
- Anno
- 2010-01-01T00:00:00+01:00 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
- 10.1080/02772241003620244 (literal)
- Alternative label
Barra Caracciolo A, Bottoni P, Grenni P (2010)
Fluorescence In Situ Hybridization in soil and water ecosystems: an useful method for studying the effect of xenobiotics on bacterial community structure
in Toxicological and environmental chemistry (Print)
(literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- Barra Caracciolo A, Bottoni P, Grenni P (literal)
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- http://dx.doi.org/10.1080/02772241003620244 (literal)
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- BOTTONI P.: Istituto Superiore di Sanita`, Viale Regina Elena 299 - 00166 Rome, Italy
Barra Caracciolo A, Grenni P: IRSA-CNR (literal)
- Titolo
- Fluorescence In Situ Hybridization in soil and water ecosystems: an useful method for studying the effect of xenobiotics on bacterial community structure (literal)
- Abstract
- The ability of soil and groundwater ecosystems to recover from chemical
contamination is primarily dependent on the presence of a microbial
community which has the ability to remove it. Nevertheless, there has been
a little research into these communities because it is strictly dependent
on methods capable of identifying and characterizing their community
structure and functioning. The use of molecular methods makes it possible
to overcome this kind of identification limitation. In this work, we applied
the fluorescence in situ hybridization (FISH) method to different samples,
such as soil and groundwater contaminated with s-triazine herbicides
(simazine or terbuthylazine) and surface water treated with the pharmaceutical
oseltamivir carboxylate (Tamiflu). We compared the bacterial
community structure in the presence/absence of these xenobiotics. The use
of 16S rRNA-targeted oligonucleotide probes, designed specifically for
the main phylogenetic levels (Archaea, Bacteria, ?-, ?-, ?-, \"-subdivision
of Proteobacteria, Planctomycetes, Gram-positive bacteria with a high or
low DNA GþC content, Cytophaga-Flavobacter-Bacteroides phylum,
and sulfate-reducing bacteria), and a DAPI stain made it possible to assess
the structure of the bacterial community and its changes in the presence
of these xenobiotics in all the ecosystems studied. (literal)
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