Detection of Meloidogyne incognita and Pochonia chlamydosporia by fluorogenic molecular probes. (Articolo in rivista)

Type
Label
  • Detection of Meloidogyne incognita and Pochonia chlamydosporia by fluorogenic molecular probes. (Articolo in rivista) (literal)
Anno
  • 2005-01-01T00:00:00+01:00 (literal)
Alternative label
  • Ciancio A., Loffredo A., Paradies F., Turturo C., Finetti Sialer M. (2005)
    Detection of Meloidogyne incognita and Pochonia chlamydosporia by fluorogenic molecular probes.
    in Bulletin OEPP (Print)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Ciancio A., Loffredo A., Paradies F., Turturo C., Finetti Sialer M. (literal)
Pagina inizio
  • 157 (literal)
Pagina fine
  • 164 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 35 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Ciancio A., Paradies F., Turturo C., Istituto per la Protezione delle Piante, CNR, Sez. di Bari, Via G. Amendola 165/A, I-70126 Bari, Italy Loffredo A., Dipartimento di Entomologia Agraria, Università degli Studi di Napoli Federico II, Napoli (Italy) Finetti Sialer M., Dipartimento di Protezione delle Piante dalle Malattie e Microbiologia Applicata, Università degli Studi, Bari (Italy) (literal)
Titolo
  • Detection of Meloidogyne incognita and Pochonia chlamydosporia by fluorogenic molecular probes. (literal)
Abstract
  • Fluorescent molecular probes were applied for detection of the plant parasitic nematode Meloidogyne incognita and the nematode-egg parasitic fungus Pochonia chlamydosporia var. chlamydosporia. A region in the M. incognitar DNA including ITS2 was selected for amplification and recognition with a real-time PCR assay, based on a combination of three specific motifs, each recognized by a specific fluorescent probe. Similarly, a Scorpion probe was designed for the RT-PCR quantification of P. c. chlamydosporia. For this purpose, the ITS-2 rDNA gene of the fungus was sequenced from a number of Italian isolates. A conserved region unique for P. c. chlamydosporia found in the ITS-2 rDNA gene was used. The probes allowed recognition of single juveniles of M. incognita and of the mycelium- or soil-extracted fungal DNA. The potentialities of the detection procedures are discussed. (literal)
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