ASSOCIATION OF METALS (MERCURY, CADMIUM AND ZINC) WITH METALLOTHIONEIN-LIKE PROTEINS IN STORAGE ORGANS OF STRANDED DOLPHINS FROM THE MEDITERRANEAN SEA (SOUTHERN ITALY) (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• ASSOCIATION OF METALS (MERCURY, CADMIUM AND ZINC) WITH METALLOTHIONEIN-LIKE PROTEINS IN STORAGE ORGANS OF STRANDED DOLPHINS FROM THE MEDITERRANEAN SEA (SOUTHERN ITALY) (Articolo in rivista) (literal)

Anno

• 2004-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1039/b315685k (literal)

Alternative label

• A., Decataldo, A., Di Leo, S., Giandomenico and N., Cardellicchio (2004)
  ASSOCIATION OF METALS (MERCURY, CADMIUM AND ZINC) WITH METALLOTHIONEIN-LIKE PROTEINS IN STORAGE ORGANS OF STRANDED DOLPHINS FROM THE MEDITERRANEAN SEA (SOUTHERN ITALY)
  in Journal of environmental monitoring (Print); Royal Society of Chemistry, Cambridge (Regno Unito)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• A., Decataldo, A., Di Leo, S., Giandomenico and N., Cardellicchio (literal)

Pagina inizio

• 361 (literal)

Pagina fine

• 367 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url

• http://www.rsc.org/publishing/journals/EM/about.asp (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 6 (literal)

Rivista

• Journal of environmental monitoring (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali

• 7 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 4 (literal)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• CNR, Istituto per l'Ambiente Marino Costiero, Taranto (literal)

Titolo

• ASSOCIATION OF METALS (MERCURY, CADMIUM AND ZINC) WITH METALLOTHIONEIN-LIKE PROTEINS IN STORAGE ORGANS OF STRANDED DOLPHINS FROM THE MEDITERRANEAN SEA (SOUTHERN ITALY) (literal)

Abstract

• Selected toxic and essential metals (mercury, Hg; cadmium, Cd; and zinc, Zn) were determined in the liver and in the kidney collected from 13 dolphins (Stenella coeruleoalba and Tursiops truncatus) stranded along the southern coasts of Italy from 1991 to 1999. Liver samples were also analysed for their methyl mercury (MeHg) and selenium (Se) contents. For subcellular fractionation, liver and kidney samples were homogenized in Tris-HCl buffer; after centrifugation, the supernatant (cytosol) was separated from pellets (insoluble fraction), heated at 80 uC for 10 min and centrifuged in order to separate the precipitate containing the thermolabile high molecular weight proteins. The cytosol heat-stable fraction, including metallothionein-like proteins (MTLPs), was then purified by gel filtration chromatography on Sephadex G-75 column. The three subcellular fractions collected (insoluble fraction, thermolabile fraction and purified heat-stable fraction) were analysed for their Hg, Cd and Zn contents. The analytical results confirm previous similar studies on toothed whales in showing that: (1) in the liver, as well as in the kidney, Hg was mainly found in the insoluble fraction, therefore, metallothioneins seem to have no role in the Hg detoxification; (2) in the liver, the molar ratio between Se and inorganic Hg was very close to 1; this suggests that the final compound of MeHg detoxification could be HgSe (tiemannite); (3) in almost all the samples, Cd and Zn were detected in the purified heat-stable fraction (including MTLPs). The mechanism of Cd detoxification and Zn homeostasis is also discussed. (literal)

Editore

• Royal Society of Chemistry (Editore)

Prodotto di

• Institute for coastal marine environment (IAMC) (Istituto)
• Fascia Costiera e Ambienti di Transizione: analisi strutturale e funzionale delle componenti biotiche ed abiotiche degli ecosistemi per lo sviluppo sostenibile delle attività (TA.P04.002.001) (Modulo)

Autore CNR

• ANTONELLA DI LEO (Persona)
• NICOLA CARDELLICCHIO (Persona)
• SANTINA GIANDOMENICO (Persona)

Insieme di parole chiave

• Keywords of "ASSOCIATION OF METALS (MERCURY, CADMIUM AND ZINC) WITH METALLOTHIONEIN-LIKE PROTEINS IN STORAGE ORGANS OF STRANDED DOLPHINS FROM THE MEDITERRANEAN SEA (SOUTHERN ITALY)" (Insieme di parole chiave)

Incoming links:

Prodotto

• Institute for coastal marine environment (IAMC) (Istituto)
• Fascia Costiera e Ambienti di Transizione: analisi strutturale e funzionale delle componenti biotiche ed abiotiche degli ecosistemi per lo sviluppo sostenibile delle attività (TA.P04.002.001) (Modulo)

Autore CNR di

• ANTONELLA DI LEO (Persona)
• NICOLA CARDELLICCHIO (Persona)
• SANTINA GIANDOMENICO (Persona)

Editore di

• Royal Society of Chemistry (Editore)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Journal of environmental monitoring (Rivista)

Insieme di parole chiave di

• Keywords of "ASSOCIATION OF METALS (MERCURY, CADMIUM AND ZINC) WITH METALLOTHIONEIN-LIKE PROTEINS IN STORAGE ORGANS OF STRANDED DOLPHINS FROM THE MEDITERRANEAN SEA (SOUTHERN ITALY)" (Insieme di parole chiave)