Salvaguardia, caratterizzazione e valorizzazione dell’'antico agro-ecotipo pugliese “Lenticchia di Altamura” (Articolo in rivista)

Type
Label
  • Salvaguardia, caratterizzazione e valorizzazione dell’'antico agro-ecotipo pugliese “Lenticchia di Altamura” (Articolo in rivista) (literal)
Anno
  • 2006-01-01T00:00:00+01:00 (literal)
Alternative label
  • Laghetti G., Volpe N., Sonnante G., Pignone D., Sonnante G. (2006)
    Salvaguardia, caratterizzazione e valorizzazione dell’'antico agro-ecotipo pugliese “Lenticchia di Altamura”
    in Italus hortus
    (literal)
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  • Laghetti G., Volpe N., Sonnante G., Pignone D., Sonnante G. (literal)
Pagina inizio
  • 467 (literal)
Pagina fine
  • 471 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 13 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
  • 2 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • CNR Igv-Bari (literal)
Titolo
  • Salvaguardia, caratterizzazione e valorizzazione dell’'antico agro-ecotipo pugliese “Lenticchia di Altamura” (literal)
Abstract
  • Since the '70s, the Institute of Plant Genetics (IGV) has been working on the safeguard of autochthonous Italian lentil agro-ecotypes in collaboration with other Italian and international institutions. This activity has led to the establishment of a rich germplasm collection of Italian lentils. Some years ago, a number of studies has been started on the characterisation of specific agro-ecotypes, particularly interesting because élite germplasm or because at risk of genetic erosion or extinction. Recently, a project on the safeguard, characterisation and reintroduction of the disappeared agroecotype from Apulia region 'Lenticchia di Altamura' (Lens culinaris Medik. ssp. macrosperma Barul.) was funded by some entrepreneurs. This ecotype is characterised by large, flat and pale green seeds. The only 10 lentil accessions collected in Altamura countryside in the past were retrieved from the germplasm bank present at the IGV. From the year 2002/2003, these accessions were evaluated for morpho-agronomic traits in two localities for two years. The highest yields were observed for populations ALT9 (5,1 q/ha) and ALT8 (4,5 q/ha), similar to the modern commercial cultivars Laird, Eston and Regular, used as controls. A low phenotypic intra-population variation was observed. The other most important agronomic descriptors detected were: time to flowering, plant height and height of lowest pod. Moreover, in the year 2003/2004, an agronomic trial with two sowing time, autumn and spring, was performed. For the spring sowing, a yield decrease of 45% was observed, whereas the harvesting time was no significantly different. Cluster analysis derived from morpho-agronomic data grouped all ecotypes but ALT3 into three clusters: (a) ALT1, ALT2 e ALT5, (b) ALT4, ALT7 e ALT9 e (c) ALT6, ALT8 e ALT10. In order to determine the level of genetic variation within and between populations, AFLP analysis was performed on 15 individuals per each of the six populations which showed bigger seeds; cultivars Laird and Eston were used as controls. Three primer combinations were tested (E-ACT+M-CAT, E-AAG+M-CTC, E-AGC+M-CAT), producing a total of 204 fragments; in the Altamura populations 197 fragments were present, 84.31% of which were polymorphic. The presence or absence of each fragment was detected and a binary matrix was constructed. These data were used to calculate some indices of genetic diversity: number of observed alleles, Nei's index of gene diversity, Nei's total genetic diversity, genetic diversity within and among populations. Results showed that, at the molecular level, most of the variation was present within populations, indicating that the six Altamura populations analysed are not well differentiated among them and could represent sub-populations recently derived from a single population. Another possible interpretation could be that there has been a certain level of geneflow among the sub-populations, due to a continuous seed exchange among farmers. (literal)
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