Combined fluorescent antibody assay and viability staining for the assessment of the physiological states of Escherichia coli in seawaters (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• Combined fluorescent antibody assay and viability staining for the assessment of the physiological states of Escherichia coli in seawaters (Articolo in rivista) (literal)

Anno

• 2003-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1046/j.1365-2672.2003.01967.x (literal)

Alternative label

• G. Caruso; M. Mancuso; E. Crisafi (2003)
  Combined fluorescent antibody assay and viability staining for the assessment of the physiological states of Escherichia coli in seawaters
  in Journal of applied microbiology (Print); Blackwell, Oxford (Regno Unito)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• G. Caruso; M. Mancuso; E. Crisafi (literal)

Pagina inizio

• 225 (literal)

Pagina fine

• 233 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#altreInformazioni

• prodotto con 11 citazioni SCOPUS, 9 citazioni ISI, 16 citazioni Google Scholar, SJR 2011: 0.864 - SJR 2009: 0.812, IF 2009: 2.098 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 95 (literal)

Rivista

• Journal of applied microbiology (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali

• 9 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 2 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#descrizioneSinteticaDelProdotto

• Water quality assessment is a topic of primary concern for the protection of public health. To deal with the need for advanced techniques for environmental monitoring, a procedure combining the fluorescent antibody with the viability staining was set up at the Institute for Marine Coastal Environment; this method represents a further development of the fluorescent antibody technique based on immune sera. The technique relies on the labelling of the cells by the fluorochromes Propidium iodide (PI) and 5-ciano-2,3-ditolyl tetrazolium chloride (CTC), markers of cells with damaged membranes and of active respiring cells, respectively. At 536 nm excitation light, dead cells (PI-positives) are visibile as red-fluorescing, so that they can be easily distinguished within the total E.coli population, which includes both viable and non viable cells and is green- fluorescing due to labelling with fluorescein isothiocianate (FITC). This new staining procedure developed in our laboratory allowed the easy discrimination of viable from dead or inactive cells that have lost their metabolic activity. Some results obtained by the application of the analytical protocol are reported in the article. (literal)

Note

• ubMe (literal)
• Google Scholar (literal)
• Scopu (literal)
• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Istituto per l'Ambiente Marino Costiero - Sez. di Messina - CNR (literal)

Titolo

• Combined fluorescent antibody assay and viability staining for the assessment of the physiological states of Escherichia coli in seawaters (literal)

Abstract

• Aims: A comparison of methods that combine the use of immune sera with specific fluorescent probes for testing viability at single cell level was performed in order to estimate different living attributes of Escherichia coli in natural seawater samples. Methods and Results: Cell culturability was assayed by plate method, respiratory activity and membrane integrity were determined by an indirect fluorescent antibody assay, combined with 5-cyano-2, 3 ditolyl tetrazolium chloride and propidium iodide, respectively. Results showed the coexistence of different physiological states within the E. coli population, of which a large fraction (46%) of cells was actively respiring. Conclusions: The methodological approach used offer interesting perspectives in water pollution monitoring, particularly when the differentiation between dead and living E. coli cells is required for a more precise assessment of the bacteriological quality of seawaters. Significance and Impact of the Study: The study suggests the importance of knowledge of the viability status of faecal bacteria in aquatic environments as a fundamental issue for the preservation of public health; the availability of rapid analytical procedures for this purpose may find significant applications in the evaluation of the sanitary risk consequent to water use. (literal)

Editore

• Blackwell (Editore)

Prodotto di

• Qualità del Mare e delle Acque Costiere: (TA.P03.003.001) (Modulo)
• Institute for coastal marine environment (IAMC) (Istituto)

Autore CNR

• ERMANNO CRISAFI (Unità di personale interno)
• MONIQUE MANCUSO (Persona)
• GABRIELLA CARUSO (Persona)

Insieme di parole chiave

• Keywords of "Combined fluorescent antibody assay and viability staining for the assessment of the physiological states of Escherichia coli in seawaters" (Insieme di parole chiave)

Incoming links:

Prodotto

• Institute for coastal marine environment (IAMC) (Istituto)
• Qualità del Mare e delle Acque Costiere: (TA.P03.003.001) (Modulo)

Autore CNR di

• ERMANNO CRISAFI (Unità di personale interno)
• GABRIELLA CARUSO (Persona)
• MONIQUE MANCUSO (Persona)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Journal of applied microbiology (Rivista)

Editore di

• Blackwell (Editore)

Insieme di parole chiave di

• Keywords of "Combined fluorescent antibody assay and viability staining for the assessment of the physiological states of Escherichia coli in seawaters" (Insieme di parole chiave)