Transgenic mice expressing an artificial zinc finger regulator targeting an endogenous gene (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Transgenic mice expressing an artificial zinc finger regulator targeting an endogenous gene (Articolo in rivista) (literal)

Anno

• 2010-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1007/978-1-60761-753-2_11 (literal)

Alternative label

• Passananti C; Corbi N; Onori A; Di Certo MG; Mattei E. (2010)
  Transgenic mice expressing an artificial zinc finger regulator targeting an endogenous gene
  in Methods in molecular biology (Clifton N.J.)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Passananti C; Corbi N; Onori A; Di Certo MG; Mattei E. (literal)

Pagina inizio

• 183 (literal)

Pagina fine

• 196 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 649 (literal)

Rivista

• Methods in molecular biology (Rivista)

Note

• Scopu (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• IBPM-CNR, Regina Elena Cancer Institute, Rome, Italy; INMN-CNR, Rome, Italy; (literal)

Titolo

• Transgenic mice expressing an artificial zinc finger regulator targeting an endogenous gene (literal)

Abstract

• Zinc finger (ZF) proteins belonging to the Cys2-His2 class provide a simple and versatile framework to design novel artificial transcription factors (ATFs) targeted to the desired genes. Our work is based on ZF ATFs engineered to up-regulate the expression level of the dystrophin-related gene utrophin in Duchenne muscular dystrophy (DMD). In particular, on the basis of the \"recognition code\" that defines specific rules between zinc finger primary structure and potential DNA-binding sites we engineered and selected a new family of artificial transcription factors, whose DNA-binding domain consists in a three zinc finger peptide called \"Jazz.\" Jazz protein binds specifically the 9 bp DNA sequence (5?-GCT-GCT-GCG-3?) present in the promoter region of both the human and mouse utrophin gene. We generated a transgenic mouse expressing Jazz protein fused to the strong transcriptional activation domain VP16 and under the control of the muscle specific promoter of the myosin light chain gene. Vp16-Jazz mice display a strong up-regulation of the utrophin at both mRNA and protein levels. To our knowledge, this represents the first example of a transgenic mouse expressing an artificial gene coding for a zinc finger-based transcription factor. © 2010 Springer Science+Business Media, LLC. (literal)

Prodotto di

• Controllo di proliferazione, differenziamento e morte cellulare (SV.P15.010.001) (Modulo)
• Institute of molecular biology and pathology (IBPM) (Istituto)

Autore CNR

• CLAUDIO PASSANANTI (Unità di personale interno)
• MARIA GRAZIA DI CERTO (Persona)
• NICOLETTA CORBI (Persona)
• ANNALISA ONORI (Persona)
• ELISABETTA MATTEI (Persona)

Incoming links:

Prodotto

• Institute of molecular biology and pathology (IBPM) (Istituto)
• Controllo di proliferazione, differenziamento e morte cellulare (SV.P15.010.001) (Modulo)

Autore CNR di

• MARIA GRAZIA DI CERTO (Persona)
• CLAUDIO PASSANANTI (Unità di personale interno)
• ELISABETTA MATTEI (Persona)
• ANNALISA ONORI (Persona)
• NICOLETTA CORBI (Persona)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Methods in molecular biology (Rivista)