Monitoring of HAB species in the Mediterranean Sea through a filter system-PCR assay detection method (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• Monitoring of HAB species in the Mediterranean Sea through a filter system-PCR assay detection method (Articolo in rivista) (literal)

Anno

• 2006-01-01T00:00:00+01:00 (literal)

Alternative label

• Penna A., Fusco G., Bertozzini E. Vila M., Garcés E., Giacobbe M.G., Lugliè A., Masò M., Galluzzi L., Magnani M. (2006)
  Monitoring of HAB species in the Mediterranean Sea through a filter system-PCR assay detection method
  in African journal of marine science (Print)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Penna A., Fusco G., Bertozzini E. Vila M., Garcés E., Giacobbe M.G., Lugliè A., Masò M., Galluzzi L., Magnani M. (literal)

Pagina inizio

• 241 (literal)

Pagina fine

• 243 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 28 (literal)

Rivista

• African journal of marine science (Rivista)

Note

• Scopu (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Università Urbino CSIC Università di Sassari (literal)

Titolo

• Monitoring of HAB species in the Mediterranean Sea through a filter system-PCR assay detection method (literal)

Abstract

• A filter system for collecting phytoplankton cells coupled to a target species-specific polymerase chain reaction (PCR) assay was performed on a spatial and temporal series of net and seawater samples as part of the Mediterranean Sea EU project Strategy (EVK-CT-2001-00046). The application of PCR allowed rapid detection of several harmful dinoflagellate species and genera, including Alexandrium spp. In this study, oligonucleotide primers targeting the 5.8S rDNA and ITS regions of the genus Alexandrium and the species A. minutum were developed. Field samples were concentrated onto filter membranes, total DNA was extracted from mixed phytoplankton populations and PCR assays were carried out with specific primers. Qualitative PCR results were compared with light and epifluorescence microscopic examinations. Results indicated that the molecular assay was able to detect harmful target cells at concentrations undetectable by microscopy. Application of this filter PCR assay to seawater samples revealed it to be a sensitive and rapid procedure for routine monitoring of field samples. Copyright © NISC Pty Ltd. (literal)

Prodotto di

• Approccio integrato per un'acquacoltura sostenibile: aspetti ambientali, qualità, benessere e applicazioni di nuove tecnologie (TA.P04.019.001) (Modulo)
• Institute for coastal marine environment (IAMC) (Istituto)

Autore CNR

• MARIA GIACOBBE (Unità di personale interno)

Insieme di parole chiave

• Parole chiave di "Monitoring of HAB species in the Mediterranean Sea through a filter system-PCR assay detection method" (Insieme di parole chiave)

Incoming links:

Prodotto

• Approccio integrato per un'acquacoltura sostenibile: aspetti ambientali, qualità, benessere e applicazioni di nuove tecnologie (TA.P04.019.001) (Modulo)
• Institute for coastal marine environment (IAMC) (Istituto)

Autore CNR di

• MARIA GIACOBBE (Unità di personale interno)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• African journal of marine science (Rivista)

Insieme di parole chiave di

• Parole chiave di "Monitoring of HAB species in the Mediterranean Sea through a filter system-PCR assay detection method" (Insieme di parole chiave)