Identification of candidate genes and expression profiles, as doping biomarkers. (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• Identification of candidate genes and expression profiles, as doping biomarkers. (Articolo in rivista) (literal)

Anno

• 2007-01-01T00:00:00+01:00 (literal)

Alternative label

• Paparini A., Impagnatiello F., Pistilli A., Rinaldi M.1, Gianfranceschi G., Signori E.2, Stabile A.M,, Fazio V.3, Rende M., Romano Spica V. (2007)
  Identification of candidate genes and expression profiles, as doping biomarkers.
  
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Paparini A., Impagnatiello F., Pistilli A., Rinaldi M.1, Gianfranceschi G., Signori E.2, Stabile A.M,, Fazio V.3, Rende M., Romano Spica V. (literal)

Pagina inizio

• 303 (literal)

Pagina fine

• 314 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 19(4) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#note

• La rivista è presente in PubMed (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• 1,2,3 = CNR, Institute of Neurobiology and Molecular Medicine, Reserch Area of Tor Vergata, Rome, Italy; Dipartimento di Scienze della Salute, Unità di Salute Pubblica, Istituto Universitario Scienze Motorie, Roma (literal)

Titolo

• Identification of candidate genes and expression profiles, as doping biomarkers. (literal)

Abstract

• Administration of prohibited substances to enhance athletic performance represents an emerging medical, social, ethical and legal issue. Traditional controls are based on direct detection of substances or their catabolites. However out-of-competition doping may not be easily revealed by standard analytical methods. Alternative indirect control strategies are based on the evaluation of mid- and long-term effects of doping in tissues. Drug-induced long-lasting changes of gene expression may be taken as effective indicators of doping exposure. To validate this approach, we used real-time PCR to monitor the expression pattern of selected genes in human haematopoietic cells exposed to nandrolone, insulin-like growth factor I (IGF-I) or growth hormone (GH). Some candidate genes were found significantly and consistently modulated by treatments. Nandrolone up-regulated AR, ESR2 and PGR in K562 cells, and SRD5A1, PPARA and JAK2 in Jurkat cells; IGF-I up-regulated EPOR and PGR in HL60 cells, and SRD5A1 in Jurkat; GH up-regulated SRD5A1 and GHR in K562. GATA1 expression was down-regulated in IGF-1-treated HL60, ESR2 was down-regulated in nandrolone-treated Jurkat, and AR and PGR were down-regulated in GH-treated Jurkat. This pilot study shows the potential of molecular biology-based strategies in anti-doping controls. (literal)

Prodotto di

• Istituto di neurobiologia e medicina molecolare (INMM) (Istituto)
• Target Molecolari, Modelli preclinici e Immunoterapia (ME.P03.006.001) (Modulo)

Autore CNR

• Vito Michele Fazio (Unità di personale esterno)
• EMANUELA SIGNORI (Unità di personale interno)
• MONICA RINALDI (Unità di personale interno)

Incoming links:

Autore CNR di

• EMANUELA SIGNORI (Unità di personale interno)
• MONICA RINALDI (Unità di personale interno)
• Vito Michele Fazio (Unità di personale esterno)

Prodotto

• Istituto di neurobiologia e medicina molecolare (INMM) (Istituto)
• Target Molecolari, Modelli preclinici e Immunoterapia (ME.P03.006.001) (Modulo)