Performance evaluation of an in-house human immunodeficiency virus type-1 protease-reverse transcriptase genotyping assay in Cameroon. (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• Performance evaluation of an in-house human immunodeficiency virus type-1 protease-reverse transcriptase genotyping assay in Cameroon. (Articolo in rivista) (literal)

Anno

• 2011-01-01T00:00:00+01:00 (literal)

Alternative label

• Fokam J, Salpini R, Santoro MM, Cento V, D'Arrigo R, Gori C, Perno CF, Colizzi V, Nanfack A, Gwom LC, *Cappelli G, Takou D. (2011)
  Performance evaluation of an in-house human immunodeficiency virus type-1 protease-reverse transcriptase genotyping assay in Cameroon.
  in Archives of virology
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Fokam J, Salpini R, Santoro MM, Cento V, D'Arrigo R, Gori C, Perno CF, Colizzi V, Nanfack A, Gwom LC, *Cappelli G, Takou D. (literal)

Pagina inizio

• 1235 (literal)

Pagina fine

• 1243 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 156 (literal)

Rivista

• Archives of virology (Rivista)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• - CIRCB: Chantal BIYA International Research Centre for Research on HIV/AIDS Prevention and Management, Yaoundé, Cameroon. - *Institute of Translational Pharmacology, National Research Council, Rome, Italy. (literal)

Titolo

• Performance evaluation of an in-house human immunodeficiency virus type-1 protease-reverse transcriptase genotyping assay in Cameroon. (literal)

Abstract

• Most commercial HIV-1 genotyping assays are hampered by high cost in resource-limited settings. Moreover, their performance might be influenced over time by HIV genetic heterogeneity and evolution. An in-house genotyping protocol was developed, and its sequencing performance and reproducibility were compared to that of ViroSeq™. One hundred ninety plasma samples from HIV-1-infected subjects in Cameroon, a resource-limited setting with a high HIV genetic variability, were processed for pol gene sequencing with an in-house protocol, ViroSeq™, or both. Only non-B subtypes were found. The in-house sequencing performance was 98.7% against 92.1% with ViroSeq™. Among 36 sequence pairs obtained using both assays, the overall rate of discordant amino acid positions was negligible (0.24%). With its high sensitivity and reproducibility, as well as its affordable cost (about half of ViroSeq™: 92 euros vs. 217 euros), this in-house assay is a suitable alternative for HIV-1 genotyping in resource-limited and/or in high-genetic-diversity settings. (literal)

Prodotto di

• Ricerca traslazionale ed outcome nelle patologie infiammatorie e degenerative (ME.P04.009.001) (Modulo)

Autore CNR

• GIULIA CAPPELLI (Unità di personale interno)

Incoming links:

Autore CNR di

• GIULIA CAPPELLI (Unità di personale interno)

Prodotto

• Ricerca traslazionale ed outcome nelle patologie infiammatorie e degenerative (ME.P04.009.001) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Archives of virology (Rivista)