Cord Blood CD133 Cells Define an OV6-Positive Population That Can Be Differentiated In Vitro into Engraftable Bipotent Hepatic Progenitors. (Articolo in rivista)

Type
Label
  • Cord Blood CD133 Cells Define an OV6-Positive Population That Can Be Differentiated In Vitro into Engraftable Bipotent Hepatic Progenitors. (Articolo in rivista) (literal)
Anno
  • 2011-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1089/scd.2010.0545 (literal)
Alternative label
  • Crema A, Ledda M, De Carlo F, Fioretti D, Rinaldi M, Marchese R, Sanchez M, Giuliani M, Arena V, Durrbach A, Brunetti E, Haas C, Ponzetto A, Lisi A, Carloni G. (2011)
    Cord Blood CD133 Cells Define an OV6-Positive Population That Can Be Differentiated In Vitro into Engraftable Bipotent Hepatic Progenitors.
    in Stem cells and development; Mary Ann Liebert Inc., New Rochelle (Stati Uniti d'America)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Crema A, Ledda M, De Carlo F, Fioretti D, Rinaldi M, Marchese R, Sanchez M, Giuliani M, Arena V, Durrbach A, Brunetti E, Haas C, Ponzetto A, Lisi A, Carloni G. (literal)
Pagina inizio
  • 2009 (literal)
Pagina fine
  • 2021 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#altreInformazioni
  • 2011 La pubblicazione è stata inclusa nell'edizione \"CNR Highlights\" 2010-2011, una selezione di 220 pubblicazioni scientifiche, sulla base dell'impact factor e del contributo particolarmente innovativo, tra le 15.000 pubblicazioni prodotte dal CNR negli anni 2010-2011. 2012 Fotografia in home page sul sito del CNR, Stem Cell Dev. 2011, vol. 20, pp. 2009-21. (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 20 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
  • 11 (literal)
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Institute of Translational Pharmacology, Department of Medicine, National Research Council (CNR), Rome, Italy. Research Center, FBF S. Peter Hospital, Rome, Italy. Department of Cell Biology and Neuroscience ISS, Rome, Italy. Inserm UMR 1014, Paul Brousse Hospital, Villejuif, France. Department of Pathology, ''Sacro Cuore'' Catholic University, ''A. Gemelli'' Hospital, Rome, Italy. Department of Neonatology, FBF S. Peter Hospital, Rome, Italy. Department of Internal Medicine, Turin University, Turin, Italy. (literal)
Titolo
  • Cord Blood CD133 Cells Define an OV6-Positive Population That Can Be Differentiated In Vitro into Engraftable Bipotent Hepatic Progenitors. (literal)
Abstract
  • Cell therapy represents the most promising alternative strategy for end-stage liver diseases and hepatic progenitors are the best candidates. We have identified a reservoir of immature hepatic precursors within human cord blood, which can derive engraftable bipotent progenitors. We isolated a stem cell subset CD133+/CD34+/OV6(low) expressing a surface-marker profile consistent with that of fetal liver cells. Upon induction of hepatic commitment by a medium containing cytokines and factors involved in vivo oval-cell activation, a heterogeneous cell population displaying characteristics of functional oval-cell-like bipotent hepatic progenitors was obtained. The cells expressed markers of hepatocytes and cholangiocytes and were highly enriched in OV6, c-Met, c-Kit, and Thy-1. They also displayed liver functional activity as glycogen storage, urea production, albumin secretion, and inducible CyP2B6 activity. When injected into liver-damaged severe-combined immunodeficient mice, induced bipotent hepatic progenitors appropriately engrafted livers of recipient animals, where they formed clusters of human-derived cells expressing human leucocyte antigen-class I, Hep-Par1, and OV6 antigens. Human-specific albumin, alpha-fetoprotein, and cytokeratin 19 were also expressed. In transplanted animals, AST serum levels showed a significative reduction with regard to controls. This human model for in vitro progenitor-cell activation may provide a powerful tool for elucidating the pathways and synergies that regulate this complex process and can represent a valuable source, exploitable for liver cell-based therapies and regenerative medicine. (literal)
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