MicroRNA-155 Targets the SKI Gene in Human Melanoma Cell Lines (Articolo in rivista)

Type
Label
  • MicroRNA-155 Targets the SKI Gene in Human Melanoma Cell Lines (Articolo in rivista) (literal)
Anno
  • 2011-01-01T00:00:00+01:00 (literal)
Alternative label
  • Lauretta Levati, Elena Pagani, Sveva Romani, Daniele Castiglia, Eugenia Piccinni, Claudia Covaciu, Patrizia Caporaso, Sergio Bondanza, Francesca Romana Antonetti, Enzo Bonmassar, Fabio Martelli, Ester Alvino and Stefania D’Atri (2011)
    MicroRNA-155 Targets the SKI Gene in Human Melanoma Cell Lines
    in Pigment cell & melanoma research (Print)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Lauretta Levati, Elena Pagani, Sveva Romani, Daniele Castiglia, Eugenia Piccinni, Claudia Covaciu, Patrizia Caporaso, Sergio Bondanza, Francesca Romana Antonetti, Enzo Bonmassar, Fabio Martelli, Ester Alvino and Stefania D’Atri (literal)
Rivista
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Laboratory of Molecular Oncology, Laboratory of Vascular Pathology, Laboratory of Molecular and Cell Biology, and Laboratory of Tissue Engineering and Cutaneous Physiopathology, Istituto Dermopatico dell’Immacolata-IRCCS, Via dei Monti di Creta 104, 00167 Rome, Italy; Dermatological Department, University of Oradea, Str. Universitatii 1-3, 410546 Oradea, Romania; Department of Neuroscience, School of Medicine, University of Rome “Tor Vergata”, Via Montpellier 1, 00133 Rome, Italy; Institute of Translational Pharmacology, National Council of Research, Via Fosso del Cavaliere 100, 00133 Rome, Italy. (literal)
Titolo
  • MicroRNA-155 Targets the SKI Gene in Human Melanoma Cell Lines (literal)
Abstract
  • The SKI protein is a transcriptional co-regulator over-expressed in melanoma. Experimentally induced down-regulation of SKI inhibits melanoma cell growth in vitro and in vivo. Micro-RNAs (miRNAs) negatively modulate gene expression, and have been implicated in oncogenesis. We previously showed that microRNA-155 (miR-155) is down-regulated in melanoma cells as compared with normal melanocytes, and that its ectopic expression impairs proliferation and induces apoptosis. Here, we investigated whether miR-155 could mediate melanoma growth inhibition via SKI gene silencing. Luciferase reporter assays demonstrated that miR-155 interacted with SKI 3’UTR and impaired gene expression. Transfection of melanoma cells with miR-155 reduced SKI levels, while inhibition of endogenous miR-155 up-regulated SKI expression. Specifically designed small interfering RNAs reduced SKI expression and inhibited proliferation. However, melanoma cells over-expressing a 3’UTR-deleted SKI were still susceptible to the antiproliferative effect of miR-155. Our data demonstrate for the first time that SKI is a target of miR-155 in melanoma. However, impairment of SKI expression is not the leading mechanism involved in the growth-suppressive effect of miR-155 (literal)
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