Delivery and Subcellular Targeting of Dendrimer-Based Fluorescent pH Sensors in Living Cells (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• Delivery and Subcellular Targeting of Dendrimer-Based Fluorescent pH Sensors in Living Cells (Articolo in rivista) (literal)

Anno

• 2010-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1021/ja105689u (literal)

Alternative label

• Albertazzi L., B. Storti, L. Marchetti, and F. Beltram (2010)
  Delivery and Subcellular Targeting of Dendrimer-Based Fluorescent pH Sensors in Living Cells
  in Journal of the American Chemical Society (Print)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Albertazzi L., B. Storti, L. Marchetti, and F. Beltram (literal)

Pagina inizio

• 18158 (literal)

Pagina fine

• 18167 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 132 (literal)

Rivista

• Journal of the American Chemical Society (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali

• 10 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 51 (literal)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• 1. Scuola Normale Super Pisa, NEST, I-56127 Pisa, Italy 2. CNR, Ist Nanosci, I-56127 Pisa, Italy 3. IIT NEST, Ctr Nanotechnol Innovat, I-56127 Pisa, Italy (literal)

Titolo

• Delivery and Subcellular Targeting of Dendrimer-Based Fluorescent pH Sensors in Living Cells (literal)

Abstract

• Synthesis and targeted delivery of dendrimer-based fluorescent biosensors in living HeLa cells are reported. Following electroporation dendrimers are shown to display specific subcellular localization depending on their size and surface charge and this property is preserved when they are functionalized with sensing moieties. We analyze the case of double dendrimer conjugation with pH-sensitive and pH-insensitive molecules leading to the realization of ratiometric pH sensors that are calibrated in vitro and in living cells. By tuning the physicochemical properties of the dendrimer scaffold sensors can be targeted to specific cellular compartments allowing selective pH measurements in different organelles in living cells. In order to demonstrate the modularity of this approach we present three different pH sensors with tuned H(+) affinity by appropriately choosing the pH-sensitive dye. We argue that the present methodology represents a general approach toward the realization of targetable ratiometric sensors suitable to monitor biologically relevant ions or molecules in living cells. (literal)

Prodotto di

• Progettazione ed applicazioni di Nanobiotecnologie (MD.P06.022.002) (Modulo)

Autore CNR

• BARBARA STORTI (Unità di personale esterno)
• FABIO BELTRAM (Persona)
• LORENZO ALBERTAZZI (Persona)

Insieme di parole chiave

• Keywords of "Delivery and Subcellular Targeting of Dendrimer-Based Fluorescent pH Sensors in Living Cells" (Insieme di parole chiave)

Incoming links:

Autore CNR di

• BARBARA STORTI (Unità di personale esterno)
• FABIO BELTRAM (Persona)
• LORENZO ALBERTAZZI (Persona)

Prodotto

• Progettazione ed applicazioni di Nanobiotecnologie (MD.P06.022.002) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Journal of the American Chemical Society (Rivista)

Insieme di parole chiave di

• Keywords of "Delivery and Subcellular Targeting of Dendrimer-Based Fluorescent pH Sensors in Living Cells" (Insieme di parole chiave)