http://www.cnr.it/ontology/cnr/individuo/prodotto/ID58943

Dendrimer internalization and intracellular trafficking in living cells (Articolo in rivista)

Type

Articolo in rivista (Classe)
Prodotto della ricerca (Classe)

Label

Dendrimer internalization and intracellular trafficking in living cells (Articolo in rivista) (literal)

Anno

2010-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

10.1021/mp9002464 (literal)

Alternative label

Albertazzi L., M. Serresi, A. Albanese, and F. Beltram (2010)
Dendrimer internalization and intracellular trafficking in living cells
in Molecular pharmaceutics (Print)
(literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

Albertazzi L., M. Serresi, A. Albanese, and F. Beltram (literal)

Pagina inizio

680 (literal)

Pagina fine

688 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

7 (literal)

Rivista

Molecular pharmaceutics (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali

9 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

3 (literal)

Note

ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

1. Scuola Normale Super Pisa, NEST, I-56127 Pisa, Italy 2. CNR, Ist Nanosci, I-56127 Pisa, Italy 3. Ctr Nanotechnol Innovat, IIT NEST, I-56127 Pisa, Italy (literal)

Titolo

Dendrimer internalization and intracellular trafficking in living cells (literal)

Abstract

The ability of dendrimers to cross cell membranes is of much interest for their application in drug and gene delivery. Recent studies demonstrate that dendrimers are capable to enter cells by endocytosis, but the intracellular pathway following their internalization remains controversial. In this study we use confocal fluorescence microscopy to elucidate the intracellular trafficking properties of PAMAM dendrimers with high spatial and temporal resolution in living HeLa cells. Macromolecules of different chemical functionality (neutral, cationic and lipidated), size (from G2 up to G6) and surface charge are investigated and their internalization properties correlated with the molecular structure. Toxicity and internalization data are discussed that allow the identification of dendrimers maximizing intracellular uptake with the minimum effect on cell viability. Time-lapse imaging and colocalization assays with fluorescent biomarkers for endocytic vesicles demonstrate that dendrimers are internalized by both clathrin-dependent endocytosis and macropinocytosis and are eventually delivered to the lysosomal compartment. Moreover we analyzed the uptake of dendrimers in additional cell lines of practical interest for therapeutic purposes. These measurements together with a direct comparison with TAT peptides demonstrate that PAMAM dendrimers possess similar properties to these widely used cell-penetrating peptides and thanks to their chemical tunability may represent a valid alternative for drug and gene delivery. (literal)

Prodotto di