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Modular plastic chip for one-shot human papillomavirus diagnostic analysis (Articolo in rivista)

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Modular plastic chip for one-shot human papillomavirus diagnostic analysis (Articolo in rivista) (literal)

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Vecchio G., Sabella S., Tagliaferro L., Menegazzi P., Di Bello M. P., Brunetti V., Cingolani R., Rinaldi R., Pompa P. P. (2010)
Modular plastic chip for one-shot human papillomavirus diagnostic analysis
in Analytical biochemistry (Print)
(literal)

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Vecchio G., Sabella S., Tagliaferro L., Menegazzi P., Di Bello M. P., Brunetti V., Cingolani R., Rinaldi R., Pompa P. P. (literal)

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Acknowledgement: The authors gratefully acknowledge S. Shiv Shankar for useful discussion and E. Perrone and V. Fiorelli for expert technical assistance. This work was supported by the Italian Ministry of Research through the MIUR \"FIRB\" project (RBLA03ER38_001). (literal)

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1. Italian Inst Technol, Ctr Biomol Nanotechnol, I-73010 Lecce, Italy (Sabella S., Cingolani R., Pompa P. P.); 2. Univ Salento, CNR, INFM, Natl Nanotechnol Lab,Scuola Super ISUFI, I-73100 Lecce, Italy (Vecchio G., Di Bello M. P., Brunetti V., Rinaldi R.); 3. Pignatelli Lab, Dept Virol & Mol Biol, I-73100 Lecce, Italy (Tagliaferro L., Menegazzi P.). (literal)

Titolo

Modular plastic chip for one-shot human papillomavirus diagnostic analysis (literal)

Abstract

In this article, we report the design and development of a plastic modular chip suitable for one-shot human papillomavirus (HPV) diagnostics, namely detection of the viral presence and relative genotyping, by two sequential steps performed directly on the same device. The device is composed of two modular and disposable plastic units that can be assembled or used separately. The first module is represented by a polydimethylsiloxane (PDMS) microreactor that is exploited for real-time polymerase chain reaction (PCR) and, thus, is suitable for detecting the presence of virus. The second unit is a PDMS microwell alpha-ray that allows virus genotyping by a colorimetric assay, based on DNA hybridization technology developed on plastic, requiring simple inspection by the naked eye. The two modules can be easily coupled to reusable hardware, enabling the heating/cooling processes and the real-time detection of HPV. By coupling real-time assay and colorimetric genotyping on the same chip, the assembled device may provide a low-cost tool for HPV diagnostics, thereby favoring the prediction of cancer risk in patients. (C) 2009 Elsevier Inc. All rights reserved. (literal)

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