Dynamic light scattering and atomic force microscopy imaging on fragments of Beta-connectin from human cardiac muscle (Articolo in rivista)

Type
Label
  • Dynamic light scattering and atomic force microscopy imaging on fragments of Beta-connectin from human cardiac muscle (Articolo in rivista) (literal)
Anno
  • 2008-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1103/PhysRevE.77.021910 (literal)
Alternative label
  • S. Marchetti (1,2); F. Sbrana (1,3); R. Raccis (1); L. Lanzi (1); C. M. C. Gambi (1,2); M. Vassalli (3,4); B. Tiribilli (3,4); A. Pacini (5); A. Toscano (5) (2008)
    Dynamic light scattering and atomic force microscopy imaging on fragments of Beta-connectin from human cardiac muscle
    in Physical review. E, Statistical, nonlinear, and soft matter physics (Print)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • S. Marchetti (1,2); F. Sbrana (1,3); R. Raccis (1); L. Lanzi (1); C. M. C. Gambi (1,2); M. Vassalli (3,4); B. Tiribilli (3,4); A. Pacini (5); A. Toscano (5) (literal)
Pagina inizio
  • 021910 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 77 (literal)
Rivista
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  • Lavoro selezionato dall'APS per la pubbl. online su Virtual Journal of Biological Physics Research, 15 febbraio 2008. American Physical Society (APS). (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali
  • 11 (literal)
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  • 2 (literal)
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • 1) Department of Physics and CNISM, University of Florence, Via G. Sansone 1, 50019 Sesto Fiorentino, Florence, Italy 2) CRS-Soft Matter (CNR-INFM), Università di Roma \"La Sapienza,\" p. A. Moro 2, 00185 Rome, Italy 3) CSDC, Department of Physics, University of Florence, Florence, Italy 4) Complex System Institute of the National Research Council (ISC-CNR), Florence, Italy 5) Department of Anatomy, Histology, and Forensic Medicine, University of Florence, Florence, Italy (literal)
Titolo
  • Dynamic light scattering and atomic force microscopy imaging on fragments of Beta-connectin from human cardiac muscle (literal)
Abstract
  • In order to investigate the protein folding-unfolding process, dynamic light scattering (DLS) and atomic force microscopy (AFM) imaging were used to study two fragments of the muscle cardiac protein beta-connectin, also known as titin. Both fragments belong to the I band of the sarcomer, and they are composed of four domains from I27 to I30 (tetramer) and eight domains from I27 to I34 (octamer). DLS measurements provide the size of both fragments as a function of temperature from 20 up to 86 °C, and show a thermal denaturation due to temperature increase. AFM imaging of both fragments in the native state reveals a homogeneous and uniform distribution of comparable structures. The DLS and AFM techniques turn out to be complementary for size measurements of the fragments and fragment aggregates. An unexpected result is that the octamer folds into a smaller structure than the tetramer and the unfolded octamer is also smaller than the unfolded tetramer. This feature seems related to the significance of the hydrophobic interactions between domains of the fragment. The longer the fragment, the more easily the hydrophobic parts of the domains interact with each other. The fragment aggregation behavior, in particular conditions, is also revealed by both DLS and AFM as a process that is parallel to the folding-unfolding transition. (literal)
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