Generation of virus-like particles in plants expressing the capsid protein of Cymbidium Ringspot Virus (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• Generation of virus-like particles in plants expressing the capsid protein of Cymbidium Ringspot Virus (Articolo in rivista) (literal)

Anno

• 2011-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.4454/jpp.v93i3.1247 (literal)

Alternative label

• Rubino L.; De Stradis A.; Russo M.; Martelli G.P. (2011)
  Generation of virus-like particles in plants expressing the capsid protein of Cymbidium Ringspot Virus
  in Journal of plant pathology (Print)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Rubino L.; De Stradis A.; Russo M.; Martelli G.P. (literal)

Pagina inizio

• 751 (literal)

Pagina fine

• 756 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 93 (literal)

Rivista

• Journal of plant pathology (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#note

• IMPACT FACTOR RIVISTA: 1,054 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#descrizioneSinteticaDelProdotto

• Pubblicazione (literal)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• 1 Università degli Studi Aldo Moro, Bari IVV Unità Organizzativa di Supporto Sede di Bari (literal)

Titolo

• Generation of virus-like particles in plants expressing the capsid protein of Cymbidium Ringspot Virus (literal)

Abstract

• Agroinfiltration of Nicotiana benthamiana leaves with a construct expressing the coat protein of Cymbidium ringspot virus (CyRSV) resulted in the production of virus-like particles (VLPs) which showed a preferential localization within mitochondria with an apparently intact bounding membrane. VLPs were either non penetrated or penetrated by the negative stain. The former had the same outward aspect and size of wild type CymRSV and were assumed to encapsidate the CP messanger RNA, which was recovered from virus preparations purified from agroinfiltrated tissues. In addition, VLPs were shown to be able to encapsidate tombusviral satellite RNAs. Immunoblot analysis of dissociated VLPs showed that they were made up of a protein indistinguishable from the native CymRSV CP. A mitochondrial targeting signal was identified in the N-terminal region of CymRSV CP at amino acid (aa) position 10-27. The fusion protein CPMyc, containing the 10-aa Myc epitope fused to the CymRSV CP C-terminus, formed VLPs that were decorated by a Myc-specific antiserum. (literal)

Prodotto di

• Interazioni biologiche e molecolari delle piante con virus e agenti patogeni virus-simili (AG.P01.010.001) (Modulo)

Autore CNR

• LUISA RUBINO (Unità di personale interno)
• ANGELO DE STRADIS (Unità di personale interno)
• MARCELLO RUSSO (Unità di personale interno)

Insieme di parole chiave

• Keywords of "Generation of virus-like particles in plants expressing the capsid protein of Cymbidium Ringspot Virus" (Insieme di parole chiave)

Incoming links:

Autore CNR di

• ANGELO DE STRADIS (Unità di personale interno)
• MARCELLO RUSSO (Unità di personale interno)
• LUISA RUBINO (Unità di personale interno)

Prodotto

• Interazioni biologiche e molecolari delle piante con virus e agenti patogeni virus-simili (AG.P01.010.001) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Journal of plant pathology (Rivista)

Insieme di parole chiave di

• Keywords of "Generation of virus-like particles in plants expressing the capsid protein of Cymbidium Ringspot Virus" (Insieme di parole chiave)