http://www.cnr.it/ontology/cnr/individuo/prodotto/ID56903

Cpkk1, MAPKK of Cryphonectria parasitica, Is Necessary for Virulence on Chestnut (Articolo in rivista)

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Cpkk1, MAPKK of Cryphonectria parasitica, Is Necessary for Virulence on Chestnut (Articolo in rivista) (literal)

Anno

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Alternative label

Rostagno L., Prodi A.(1) and Turina M. (2010)
Cpkk1, MAPKK of Cryphonectria parasitica, Is Necessary for Virulence on Chestnut
in Phytopathology
(literal)

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Titolo

Cpkk1, MAPKK of Cryphonectria parasitica, Is Necessary for Virulence on Chestnut (literal)

Abstract

The role of Cpkk1, a mitogen-activated protein kinase from Cryphonectria parasitica, was investigated by generating a number of mutant strains that overexpress, under the control of the cryparin promoter, both the wild-type protein and its allele with an extensive deletion in the catalytic domain. Furthermore, a hairpin construct was built and expressed to cause specific silencing of Cpkk1 mRNA transcripts. Specific mRNA silencing or overexpression was confirmed on both Northern and Western blot analysis. Selected C. parasitica strains with Cpkk1 either silenced or overexpressed were evaluated for their biological characteristics, including virulence on European chestnut, growth on different substrates, conidial sporulation, and resistance to cell-wall-degrading enzymes. Silencing of Cpkk1 and the overexpression of a defective Cpkk1 correlated with a marked reduction in virulence on 3-year-old chestnut trees, with no statistically significant effect on fungal growth in the various conditions tested. (literal)

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