Constitutive expression of HIV-VLPs in stably transfected insect cell line for efficient delivery system (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Constitutive expression of HIV-VLPs in stably transfected insect cell line for efficient delivery system (Articolo in rivista) (literal)

Anno

• 2010-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1016/j.vaccine.2010.07.054 (literal)

Alternative label

• M. Tagliamonte 1 ; M.L. Visciano 1 ; M.L. Tornesello 1 ; A. De Stradis ; F.M. Buonaguro 1 ; L. Buonaguro 1-3 (2010)
  Constitutive expression of HIV-VLPs in stably transfected insect cell line for efficient delivery system
  in Vaccine; ELSEVIER SCIENCE BV, PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS, AMSTERDAM (Paesi Bassi)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• M. Tagliamonte 1 ; M.L. Visciano 1 ; M.L. Tornesello 1 ; A. De Stradis ; F.M. Buonaguro 1 ; L. Buonaguro 1-3 (literal)

Pagina inizio

• 6417 (literal)

Pagina fine

• 6424 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 28 (literal)

Rivista

• Vaccine (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 39 (literal)

Note

• ISI Web of Science (WOS) (literal)
• PubMe (literal)
• Scopu (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• 1) Lab. of Molecular Biology and Viral Oncogenesis & AIDS Reference Center, Istituto Nazionale Tumori “Fond. G. Pascale”, Naples, Italy 3) Institute of Human Virology, University of Maryland School of Medicine, Baltimore, MD, USA (literal)

Titolo

• Constitutive expression of HIV-VLPs in stably transfected insect cell line for efficient delivery system (literal)

Abstract

• We have previously developed HIV-1 Pr55gag-based virus-like particles (HIV-VLPs) as presentation and delivery model using a transient Baculovirus expression system. Here we describe the establishment and characterization of stably transfected insect cell line for the constitutive and reproducible production of HIV-VLPs. The persistence of HIV gag coding gene has been verified in clonal resistant insect cells and the protein expression has been confirmed by Western blot analysis. The resulting HIV-VLPs have been evaluated by standard transmission electron microscopy and their immunogenicity has been evaluated in vivo. Our results demonstrate that this strategy is highly efficient for constitutive expression of conformational enveloped VLPs which can be employed as presentation and delivery system for pathogen as well as tumor-associated antigens. This represents, to our knowledge, the first example of stably transfected insect cell line for the constitutive production of VLPs. (literal)

Editore

• ELSEVIER SCIENCE BV, PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS (Editore)

Prodotto di

• Interazioni biologiche e molecolari delle piante con virus e agenti patogeni virus-simili (AG.P01.010.001) (Modulo)

Autore CNR

• ANGELO DE STRADIS (Unità di personale interno)

Insieme di parole chiave

• Parole chiave di "Constitutive expression of HIV-VLPs in stably transfected insect cell line for efficient delivery system" (Insieme di parole chiave)

Incoming links:

Autore CNR di

• ANGELO DE STRADIS (Unità di personale interno)

Prodotto

• Interazioni biologiche e molecolari delle piante con virus e agenti patogeni virus-simili (AG.P01.010.001) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Vaccine (Rivista)

Editore di

• ELSEVIER SCIENCE BV, PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS (Editore)

Insieme di parole chiave di

• Parole chiave di "Constitutive expression of HIV-VLPs in stably transfected insect cell line for efficient delivery system" (Insieme di parole chiave)