Pathogenicity of Alternanthera mosaic virus is affected by determinants in RNA-dependent RNA polymerase and by reduced efficacy of silencing suppression in a movement-competent TGB1 (Articolo in rivista)

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  • Pathogenicity of Alternanthera mosaic virus is affected by determinants in RNA-dependent RNA polymerase and by reduced efficacy of silencing suppression in a movement-competent TGB1 (Articolo in rivista) (literal)
Anno
  • 2010-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1099/vir.0.014977-0 (literal)
Alternative label
  • Hyoun-Sub Lim; Vaira,A.M.; Reinsel, M.D.; Bae, H.; Bailey, B.A.; Domier, L.L.; Hammond, J. (2010)
    Pathogenicity of Alternanthera mosaic virus is affected by determinants in RNA-dependent RNA polymerase and by reduced efficacy of silencing suppression in a movement-competent TGB1
    in Journal of general virology (Print)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Hyoun-Sub Lim; Vaira,A.M.; Reinsel, M.D.; Bae, H.; Bailey, B.A.; Domier, L.L.; Hammond, J. (literal)
Pagina inizio
  • 277 (literal)
Pagina fine
  • 287 (literal)
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  • 91 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#note
  • IMPACT FACTOR 2008: 3.092 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#descrizioneSinteticaDelProdotto
  • Articolo su rivista internazionale riguardante lo studio su determinanti di patogenicita’ presenti sulla RdRp e su una mutazione nella proteina TGB1 in grado di influenzare l’efficienza della soppressione del silenziamento. (literal)
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Hyoun-Sub Lim, USDA-ARS, USNA, Floral and Nursery Plants Research Unit, Beltsville, MD 20705, USA Michael D. Reinsel, USDA-ARS, USNA, Floral and Nursery Plants Research Unit, Beltsville, MD 20705, USA Hanhong Bae, USDA-ARS, PSI, Sustainable Perennial Crops Laboratory, Beltsville, MD 20705, USA Bryan A. Bailey, USDA-ARS, PSI, Sustainable Perennial Crops Laboratory, Beltsville, MD 20705, USA Leslie L. Domier, USDA-ARS, Department of Crop Sciences, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA John Hammond, USDA-ARS, USNA, Floral and Nursery Plants Research Unit, Beltsville, MD 20705, USA (literal)
Titolo
  • Pathogenicity of Alternanthera mosaic virus is affected by determinants in RNA-dependent RNA polymerase and by reduced efficacy of silencing suppression in a movement-competent TGB1 (literal)
Abstract
  • Four biologically active cDNA clones were derived from the Alternanthera mosaic virus (AltMV; genus Potexvirus) isolate, AltMV-SP, which differ in symptoms in infected Nicotiana benthamiana plants. Two clones induced necrosis and plant death; a mixture of all four clones induced milder symptoms than AltMV-SP. Replication of all clones was enhanced by a minimum of fourfold at 15 6C. A mixture of clones 4-7 (severe) and 3-1 (mild) was indistinguishable from AltMV-SP, but the ratio of 4-7 to 3-1 differed at 25 and 15 6C. RNA copy numbers of mixed infections were always below those of 4-7 alone. Determinants of symptom severity were identified in both Pol and TGB1; the mildest (4-1) and most severe (3-7) clones differed at three residues in the ‘core’ Pol domain [R(1110)P, K(1121)R, R(1255)K] and one [S(1535)P] in the C-terminal Pol domain of RNA-dependent RNA polymerase, and one in TGB1 [P(88)L]. Pol [P1110,R1121,K1255]+TGB1L(88)] always induced systemic necrosis at 15 6C. Gene exchanges of Pol and TGB1 each affected replication and symptom expression, with TGB1P(88) significantly reducing silencing suppression. The difference in silencing suppression between TGB1P(88) and TGB1L(88) was confirmed by an agroinfiltration assay. Further, co-expression of TGB1P(88) and TGB1L(88) resulted in interference in the suppression of silencing by TGB1L(88). Yeast two-hybrid analysis confirmed that TGB1P(88) and TGB1L(88) interact. These results identify a TGB1 residue that significantly affects replication and silencing suppression, but maintains full movement functions. (literal)
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