http://www.cnr.it/ontology/cnr/individuo/prodotto/ID56838
Cloning and characterization of small non-coding RNAs from grape (Articolo in rivista)
- Type
- Label
- Cloning and characterization of small non-coding RNAs from grape (Articolo in rivista) (literal)
- Anno
- 2009-01-01T00:00:00+01:00 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
- 10.1111/j.1365-313X.2009.03906.x (literal)
- Alternative label
Carra, A., Mica, E., Gambino, G., Pindo, M., Moser, C., Pe, E. , Schubert, A. (2009)
Cloning and characterization of small non-coding RNAs from grape
in Plant journal (Print)
(literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- Carra, A., Mica, E., Gambino, G., Pindo, M., Moser, C., Pe, E. , Schubert, A. (literal)
- Pagina inizio
- Pagina fine
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- IMPACT FACTOR 2008: 6.493 (literal)
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- Note
- ISI Web of Science (WOS) (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- Department of Arboriculture and Pomology, University of Torino, via L. da Vinci 44, 10095 Grugliasco, Italy,
Scuola Superiore SantAnna, Piazza Martiri della Liberta` 33, 56127 Pisa, Italy,
Genomics and Crop Biology Area, IASMA Research and Innovation Center Fondazione Edmund Mach, via E. Mach 1, 38010 San Michele allAdige, Italy (literal)
- Titolo
- Cloning and characterization of small non-coding RNAs from grape (literal)
- Abstract
- Small non-coding RNAs, including microRNAs (miRNAs) and small interfering RNAs (siRNAs), are effectors of
regulatory pathways underlying plant development, metabolism, and responses to biotic and abiotic stresses.
To address the nature and functions of these regulators in grapevine (Vitis vinifera L.), we have produced a
small RNA library from mixed-stage grape berries. Thirteen conserved miRNAs belonging to nine miRNA
families, a non-conserved miRNA, and four putative non-conserved miRNAs were isolated, and their
expression and targets are described. Experimentally validated targets of non-conserved miRNAs and putative
miRNAs included three genes encoding NB-LRR proteins and a gene encoding a heavy metal ion transport/
detoxification protein. Of the endogenous and pathogen-derived siRNAs that were also isolated, four
endogenous siRNAs mapped to genes encoding RD22-like proteins and two to a gene encoding a cytokinin
synthase. The siRNA id65 targeted the cytokinin synthase gene transcript with antisense complementarity,
and was specifically expressed in mature berries, in which, by contrast, expression of the cytokinin synthase
gene was strongly repressed. 5 RACE revealed that the transcript of this gene was processed in 21 nucleotide
increments from the id65 cleavage site, and that further cleavage was mediated by secondary siRNAs in cis.
These results indicate that grapevine miRNA- and siRNA-mediated regulatory circuits have evolved to
comprise processes associated with defence and fruit ripening, and broaden the range of small RNA-mediated
regulation, which was previously associated with auxin, ABA, gibberellins and jasmonate, to encompass
cytokinin metabolism. (literal)
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