Characterization of Bois noir isolates by RFLP of a Stolbur-specific putative membrane protein gene (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Characterization of Bois noir isolates by RFLP of a Stolbur-specific putative membrane protein gene (Articolo in rivista) (literal)

Anno

• 2009-01-01T00:00:00+01:00 (literal)

Alternative label

• D. Pacifico; A. Alma; B. Bagnoli; X. Foissac; G. Pasquini; M. Tessitori; C. Marzachì (2009)
  Characterization of Bois noir isolates by RFLP of a Stolbur-specific putative membrane protein gene
  in Phytopathology
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• D. Pacifico; A. Alma; B. Bagnoli; X. Foissac; G. Pasquini; M. Tessitori; C. Marzachì (literal)

Pagina inizio

• 711 (literal)

Pagina fine

• 715 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 99 (literal)

Rivista

• Phytopathology (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#note

• IMPACT FACTOR 2008: 2.192 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#descrizioneSinteticaDelProdotto

• Bois noir phytoplasma (BNp), widespread in wine-producing areas of Europe and endemic in France and Italy, is classified in the 16SrXII-A subgroup, whose members are referred to as Stolbur phytoplasmas. The 16S rDNA gene of Stolbur phytoplasma shows low variability, and few non-ribosomal genes are available as markers to assess variation among isolates. We used the Stolbur-specific stol-1H10 gene, encoding a putative membrane-exposed protein, to investigate genetic diversity of French and Italian BNp isolates from plants and insects. Amplification of stol-1H10 from infected grapevines, weeds and Hyalesthes obsoletus produced fragments of three sizes, and RFLP analysis divided these amplicons further into 12 profiles (V1 to V12). French BNp isolates were more variable than Italian ones, and different profiles were present in infected grapevines from France and Italy. Isolate V3, most abundant among Italian affected grapes but present among French ones, was found in one Urtica dioica sample and in all H. obsoletus collected on this species. Four Italian-specific profiles were represented among infected C. arvensis, the most frequent of which (V12) was also detected in H. obsoletus collected on this species. Most of the variability in the stol-1H10 sequence was associated with the type II on the tuf gene. (literal)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Di.Va.P.R.A. – Entomologia e Zoologia Applicate all’Ambiente “C. Vidano”, Università degli Studi di Torino, via L. da Vinci, 44, 10095, Grugliasco (TO), Italy. Istituto Sperimentale per la Zoologia Agraria, CRA, via Lanciola, 12/A, 50125 Firenze, Italy. UMR-1090 Génomique Diversité Pouvoir Pathogène, INRA, Université de Bordeaux2, 71 avenue Edouard Bourlaux BP81, F-33883 Villenave d’Ornon, France Istituto Sperimentale per la Patologia Vegetale, CRA, via C.G. Bertero, 22, I-00156 Roma, Italy. Dipartimento di Scienze e Tecnologie Fitosanitarie, Università degli Studi di Catania, Via S. Sofia, 100, I-95123 Catania, Italy. (literal)

Titolo

• Characterization of Bois noir isolates by RFLP of a Stolbur-specific putative membrane protein gene (literal)

Abstract

• Bois noir phytoplasma (BNp), widespread in wine-producing areas of Europe and endemic in France and Italy, is classified in the 16SrXII-A subgroup, whose members are referred to as Stolbur phytoplasmas. The 16S rDNA gene of Stolbur phytoplasma shows low variability, and few non-ribosomal genes are available as markers to assess variation among isolates. We used the Stolbur-specific stol-1H10 gene, encoding a putative membrane-exposed protein, to investigate genetic diversity of French and Italian BNp isolates from plants and insects. Amplification of stol-1H10 from infected grapevines, weeds, and Hyalesthes obsoletus produced fragments of three sizes, and restriction fragment length polymorphism analysis divided these amplicons further into 12 profiles (V1 to V12). French BNp isolates were more variable than Italian ones, and different profiles were present in infected grapevines from France and Italy. Isolate V3, most abundant among Italian affected grapes but present among French ones, was found in one Urtica dioica sample and in all H. obsoletus collected on this species. Four Italian-specific profiles were represented among infected Convolvulus arvensis, the most frequent of which (V12) was also detected in H. obsoletus collected on this species. Most of the variability in the stol-1H10 sequence was associated with type II on the tuf gene. (literal)

Prodotto di

• Agenti patogeni intracellulari e miglioramento genetico e sanitario finalizzato alla valorizzazione del germoplasma viticolo (AG.P04.009.001) (Modulo)

Autore CNR

• Alberto Alma (Unità di personale esterno)
• DAVIDE PACIFICO (Unità di personale esterno)
• CRISTINA MARZACHI' (Unità di personale interno)

Insieme di parole chiave

• Parole chiave di "Characterization of Bois noir isolates by RFLP of a Stolbur-specific putative membrane protein gene" (Insieme di parole chiave)

Incoming links:

Autore CNR di

• CRISTINA MARZACHI' (Unità di personale interno)
• DAVIDE PACIFICO (Unità di personale esterno)
• Alberto Alma (Unità di personale esterno)

Prodotto

• Agenti patogeni intracellulari e miglioramento genetico e sanitario finalizzato alla valorizzazione del germoplasma viticolo (AG.P04.009.001) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Phytopathology (Rivista)

Insieme di parole chiave di

• Parole chiave di "Characterization of Bois noir isolates by RFLP of a Stolbur-specific putative membrane protein gene" (Insieme di parole chiave)