Plum bark necrosis stem pitting-associated virus in different stone fruit species in Italy (Articolo in rivista)

Type
Label
  • Plum bark necrosis stem pitting-associated virus in different stone fruit species in Italy (Articolo in rivista) (literal)
Anno
  • 2005-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.4454/jpp.v87i2.908 (literal)
Alternative label
  • Amenduni T, Hobeika C, Minafra A, Boscia D, Castellano MA, Savino V (2005)
    Plum bark necrosis stem pitting-associated virus in different stone fruit species in Italy
    in Journal of plant pathology (Print)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Amenduni T, Hobeika C, Minafra A, Boscia D, Castellano MA, Savino V (literal)
Pagina inizio
  • 131 (literal)
Pagina fine
  • 134 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url
  • http://sipav.org/main/jpp/index.php/jpp/article/view/908 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 87 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
  • 2 (literal)
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Dipartimento di Protezione delle Piante e Microbiologia Applicata, Universit√† degli Studi di Bari; Istituto Agronomico Mediterraneo, Valenzano (Bari) (literal)
Titolo
  • Plum bark necrosis stem pitting-associated virus in different stone fruit species in Italy (literal)
Abstract
  • Different Prunus species from Southern Italy with stem pitting symptoms were assayed by RT-PCR for the presence of Plum bark necrosis stem pitting-associated virus (PBNSPaV). DNA of the expected size was amplified from cortical scrapings of almond, peach, plum, apricot, and sweet cherry. The test was repeated monthly on various tissues throughout a year. Different protocols for total nucleic acid extraction and RT-PCR amplification were compared. Virus particles of an Italian apricot isolate (APR-SP1) were purified and used to produce antiserum that reacted in IEM and ELISA with particles of an American PBNSPaV isolate. However, the antiserum did not detect PBNSPaV in crude plant extracts. (literal)
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