http://www.cnr.it/ontology/cnr/individuo/prodotto/ID56778
Plum bark necrosis stem pitting-associated virus in different stone fruit species in Italy (Articolo in rivista)
- Type
- Label
- Plum bark necrosis stem pitting-associated virus in different stone fruit species in Italy (Articolo in rivista) (literal)
- Anno
- 2005-01-01T00:00:00+01:00 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
- 10.4454/jpp.v87i2.908 (literal)
- Alternative label
Amenduni T, Hobeika C, Minafra A, Boscia D, Castellano MA, Savino V (2005)
Plum bark necrosis stem pitting-associated virus in different stone fruit species in Italy
in Journal of plant pathology (Print)
(literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- Amenduni T, Hobeika C, Minafra A, Boscia D, Castellano MA, Savino V (literal)
- Pagina inizio
- Pagina fine
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url
- http://sipav.org/main/jpp/index.php/jpp/article/view/908 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
- Rivista
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
- Note
- ISI Web of Science (WOS) (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- Dipartimento di Protezione delle Piante e Microbiologia Applicata, Università degli Studi di Bari; Istituto Agronomico Mediterraneo, Valenzano (Bari) (literal)
- Titolo
- Plum bark necrosis stem pitting-associated virus in different stone fruit species in Italy (literal)
- Abstract
- Different Prunus species from Southern Italy with
stem pitting symptoms were assayed by RT-PCR for the
presence of Plum bark necrosis stem pitting-associated
virus (PBNSPaV). DNA of the expected size was amplified
from cortical scrapings of almond, peach, plum,
apricot, and sweet cherry. The test was repeated
monthly on various tissues throughout a year. Different
protocols for total nucleic acid extraction and RT-PCR
amplification were compared. Virus particles of an Italian
apricot isolate (APR-SP1) were purified and used to
produce antiserum that reacted in IEM and ELISA
with particles of an American PBNSPaV isolate. However,
the antiserum did not detect PBNSPaV in crude
plant extracts. (literal)
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