http://www.cnr.it/ontology/cnr/individuo/prodotto/ID56771
Proteomics as a tool to improve investigation of substantial equivalence in genetically modified organisms: the case of a virus-resistant tomato. (Articolo in rivista)
- Type
- Label
- Proteomics as a tool to improve investigation of substantial equivalence in genetically modified organisms: the case of a virus-resistant tomato. (Articolo in rivista) (literal)
- Anno
- 2004-01-01T00:00:00+01:00 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
- 10.1002/pmic.200300540 (literal)
- Alternative label
CORPILLO, D., GARDINI, G., VAIRA, A.M.,BASSO, M., AIME, S., ACCOTTO, G.P. & FASANO, M. (2004)
Proteomics as a tool to improve investigation of substantial equivalence in genetically modified organisms: the case of a virus-resistant tomato.
in Proteomics (Weinh., Print)
(literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- CORPILLO, D., GARDINI, G., VAIRA, A.M.,BASSO, M., AIME, S., ACCOTTO, G.P. & FASANO, M. (literal)
- Pagina inizio
- Pagina fine
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
- Rivista
- Note
- ISI Web of Science (WOS) (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- CORPILLO, D., GARDINI, G.: Bioindustry Park Canavese, Colleretto Giacosa, Italy
BASSO, M., FASANO, M. :Università dellInsubria, Varese, Italy
AIME, S., Università di Torino (literal)
- Titolo
- Proteomics as a tool to improve investigation of substantial equivalence in genetically modified organisms: the case of a virus-resistant tomato. (literal)
- Abstract
- At present, the so-called substantial equivalence is the only widely accepted criterion
for deciding whether or not a transgenic food is, from an alimentary point of view, to
be considered totally correspondent to the traditional one from which it derives. Although
never exactly defined, it deals with a comparison between the chemical composition
of the two foods. A more in-depth analysis can be performed by one of the
most suitable methods that allows for the simultaneous screening of many components
without prior identification, the analysis of the proteome. As a model for testing
this kind of approach, we compared protein expression of two types of tomato plants,
having the same genetic background, except for a virus resistance trait introduced by
genetic engineering. When proteins extracted from seedlings of the two types were
analyzed by two-dimensional electrophoresis, no significant differences, either qualitative
or quantitative, were detected, indicating that in this case the expression of
major proteins was unmodified by the genetic manipulation. Fifteen proteins were
identified by peptide mass fingerprinting. (literal)
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