Development of a standard set of microsatellite reference alleles for the identification of grape cultivars (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Development of a standard set of microsatellite reference alleles for the identification of grape cultivars (Articolo in rivista) (literal)

Anno

• 2004-01-01T00:00:00+01:00 (literal)

Alternative label

• P. This, A. Jung, P. Boccacci, J. Borrego, R. Botta, L. Costantini, M. Crespan, G. S. Dangl, C. Eisenheld, F. Ferreira-Monteiro, S. Grando, J. Iban ez, T. Lacombe, V. Laucou, R. Magalhaes, C. P. Meredith, N. Dilani, E. Peterlunger, F. Regner, L. Zulini, E (2004)
  Development of a standard set of microsatellite reference alleles for the identification of grape cultivars
  in Theoretical and applied genetics
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• P. This, A. Jung, P. Boccacci, J. Borrego, R. Botta, L. Costantini, M. Crespan, G. S. Dangl, C. Eisenheld, F. Ferreira-Monteiro, S. Grando, J. Iban ez, T. Lacombe, V. Laucou, R. Magalhaes, C. P. Meredith, N. Dilani, E. Peterlunger, F. Regner, L. Zulini, E (literal)

Pagina inizio

• 1448 (literal)

Pagina fine

• 1458 (literal)

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• 109 (literal)

Rivista

• Theoretical and applied genetics (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#note

• Impact factor 2004: 2.981 (literal)

Note

• ISI Web of Science (WOS) (literal)

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• L. Costantini , S. Grando Laboratory of Molecular Genetics, Istituto Agrario S. Michele all’ Adige, Via Mach, 38010 Trento, Italy M. Crespan , N. Milani Sezione Ampelografia e Miglioramento Genetico, Istituto Sperimentale per la Viticoltura, via Casoni 13/A, 31058 Susegana (Treviso), Italy G. S. Dangl , C. P. Meredith Department of Viticulture and Enology, University of California, Davis, CA 95616, USA C. Eisenheld , F. Regner Department for Grapevine Breeding, HBLA u. BA Klosterneuburg, 2103 Langenzersdorf, Rehgraben 2, Austria F. Ferreira-Monteiro Instituto Portugues de Viticultura e Enologia (IPVE), Rua Eng. Frederico Ulrich 2650, 4470-605 Maia, Portugal R. Magalha˜ es Instituto de Ciencias Biomedicas de Abel Salazar (ICBAS), Universidade do Porto, Largo Prof. Abel Salazar, 2, 4099-003 Porto, Portugal E. Peterlunger , L. Zulini Department of Crop Production and Agricultural Technology, University of Udine, Via delle Scienze 208, 33100 Udine, Italy P. This (&) , T. Lacombe , V. Laucou Laboratory of Grape Genetics, UMR Diversite´ et Ge´nomes des Plantes Cultive´es (DGPC), Institut National de la Recherche Agronomique (INRA), 2, place P. Viala, 34060 Montpellier, France A. Jung , E. Maul Federal Centre of Breeding Research on Cultivated Plants, Institute for Grapevine Breeding Geilweilerhof, 76833 Siebeldingen, Germany J. Borrego , J. Ibanez Instituto Madrilen˜ o de Investigacio´ n Agraria y Alimentaria, Finca ‘‘El Enc1´n’’, Apartado 127, 28800 Alcala´ de Henares, Madrid, Spain (literal)

Titolo

• Development of a standard set of microsatellite reference alleles for the identification of grape cultivars (literal)

Abstract

• In order to investigate the comparability of microsatellite profiles obtained in different laboratories, ten partners in seven countries analyzed 46 grape cultivars at six loci (VVMD5, VVMD7, VVMD27, VVS2, VrZAG62, and VrZAG79). No effort was made to standardize equipment or protocols. Although some partners obtained very similar results, in other cases different absolute allele sizes and, sometimes, different relative allele sizes were obtained. A strategy for data comparison by means of reference to the alleles detected in well-known cultivars was proposed. For each marker, each allele was designated by a code based on the name of the reference cultivar carrying that allele. Thirty-three cultivars, representing from 13 to 23 alleles per marker, were chosen as references. After the raw data obtained by the different partners were coded, more than 97% of the data were in agreement. Minor discrepancies were attributed to errors, suboptimal amplification and visualization, and misscoring of heterozygous versus homozygous allele pairs. We have shown that coded microsatellite data produced in different laboratories with different protocols and conditions can be compared, and that it is suitable for the identification and SSR allele characterization of cultivars. It is proposed that the six markers employed here, already widely used, be adopted as a minimal standard marker set for future grapevine cultivar analyses, and that additional cultivars be characterized by means of the coded reference alleles presented here. The complete database is available at http://www.genres.de/eccdb/vitis/. Cuttings of the 33 reference cultivars are available on request from the Institut National de la Recherche Agronomique Vassal collection (didier.vares@ensam.inra.fr) (literal)

Prodotto di

• Agenti patogeni intracellulari e miglioramento genetico e sanitario finalizzato alla valorizzazione del germoplasma viticolo (AG.P04.009.001) (Modulo)

Autore CNR

• ROBERTO BOTTA (Unità di personale esterno)
• PAOLO BOCCACCI (Unità di personale esterno)

Insieme di parole chiave

• Keywords of "Development of a standard set of microsatellite reference alleles for the identification of grape cultivars" (Insieme di parole chiave)

Incoming links:

Autore CNR di

• ROBERTO BOTTA (Unità di personale esterno)
• PAOLO BOCCACCI (Unità di personale esterno)

Prodotto

• Agenti patogeni intracellulari e miglioramento genetico e sanitario finalizzato alla valorizzazione del germoplasma viticolo (AG.P04.009.001) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Theoretical and applied genetics (Rivista)

Insieme di parole chiave di

• Keywords of "Development of a standard set of microsatellite reference alleles for the identification of grape cultivars" (Insieme di parole chiave)