Real-time PCR for the quantitation of tomato yellow leaf curl Sardinia virus in tomato plants and in Bemisia tabaci. (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Real-time PCR for the quantitation of tomato yellow leaf curl Sardinia virus in tomato plants and in Bemisia tabaci. (Articolo in rivista) (literal)

Anno

• 2008-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1016/j.jviromet.2007.09.015 (literal)

Alternative label

• Mason, G., Caciagli, P., Accotto, G.P., Noris, E., (2008)
  Real-time PCR for the quantitation of tomato yellow leaf curl Sardinia virus in tomato plants and in Bemisia tabaci.
  in Journal of virological methods
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Mason, G., Caciagli, P., Accotto, G.P., Noris, E., (literal)

Pagina inizio

• 282 (literal)

Pagina fine

• 289 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url

• http://www.sciencedirect.com/science/article/pii/S0166093407003679 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 147 (literal)

Rivista

• Journal of virological methods (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#note

• IMPACT FACTOR 2007: 1.933 ( I.F. 2008 non é ancora stato pubblicato dall'ISI WOK ) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 2 (literal)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• MG, CP, AGP, NE IVV CNR (literal)

Titolo

• Real-time PCR for the quantitation of tomato yellow leaf curl Sardinia virus in tomato plants and in Bemisia tabaci. (literal)

Abstract

• Tomato yellow leaf curl Sardinia virus (TYLCSV) (Geminiviridae) is an important pathogen severely affecting tomato production in the Mediterranean basin. Although diagnostic protocols are available for its detection in plants and its vector Bemisia tabaci (Gennadius), suitable tools for estimating and comparing viral loads in plant and insect tissues are needed. In this paper, real-time PCR methods are described for quantitation of TYLCSV in both tomato plant and whitefly extracts. The DNA extraction method was optimised on TYLCSV-infected tomato tissue. The amount of virus was determined using specific primers and probe and standardised to the amount of DNA present in each sample, using selected endogenous tomato or Bemisia genes as internal references. The distribution of TYLCSV was relatively quantified within the four uppermost leaves of plants. An absolute estimation of the amount of TYLCSV in the first leaf below the apex was obtained. The kinetics of virus retention within different batches of viruliferous whiteflies was also analysed. The real-time PCR was 2200-fold more sensitive than membrane hybridisation, allowing detection of as few as 10 viral copies in a sample. These methods are potentially suitable for several applications, such as plant breeding for resistance, analysis of virus replication, and virus-vector interaction studies. (literal)

Prodotto di

• Interazioni biologiche e molecolari delle piante con virus e agenti patogeni virus-simili (AG.P01.010.001) (Modulo)

Autore CNR

• GIOVANNA MASON (Unità di personale interno)
• PIERO CACIAGLI (Unità di personale interno)
• GIAN PAOLO ACCOTTO (Unità di personale interno)
• EMANUELA NORIS (Unità di personale interno)

Insieme di parole chiave

• Parole chiave di "Real-time PCR for the quantitation of tomato yellow leaf curl Sardinia virus in tomato plants and in Bemisia tabaci." (Insieme di parole chiave)

Incoming links:

Autore CNR di

• GIAN PAOLO ACCOTTO (Unità di personale interno)
• PIERO CACIAGLI (Unità di personale interno)
• EMANUELA NORIS (Unità di personale interno)
• GIOVANNA MASON (Unità di personale interno)

Prodotto

• Interazioni biologiche e molecolari delle piante con virus e agenti patogeni virus-simili (AG.P01.010.001) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Journal of virological methods (Rivista)

Insieme di parole chiave di

• Parole chiave di "Real-time PCR for the quantitation of tomato yellow leaf curl Sardinia virus in tomato plants and in Bemisia tabaci." (Insieme di parole chiave)