http://www.cnr.it/ontology/cnr/individuo/prodotto/ID56733
Cymbidium ringspot virus harnesses RNA silencing to control the accumulation of virus parasite satellite RNA. (Articolo in rivista)
- Type
- Label
- Cymbidium ringspot virus harnesses RNA silencing to control the accumulation of virus parasite satellite RNA. (Articolo in rivista) (literal)
- Anno
- 2008-01-01T00:00:00+01:00 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
- 10.1128/JVI.01343-08 (literal)
- Alternative label
Pantaleo, V., Burgyan, J. (2008)
Cymbidium ringspot virus harnesses RNA silencing to control the accumulation of virus parasite satellite RNA.
in Journal of virology (Print)
(literal)
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- Pantaleo, V., Burgyan, J. (literal)
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- http://jvi.asm.org/content/82/23/11851 (literal)
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- Rivista
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- IMPACT FACTOR 2007: 5.332 (I.F. 2008 non é ancora stato pubblicato dallìISI WOK ) (literal)
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- ISI Web of Science (WOS) (literal)
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- PV: Istituto di Virologia Vegetale del CNR. BJ: Agricultural Biotechnology Center, Godollo-Hungary (literal)
- Titolo
- Cymbidium ringspot virus harnesses RNA silencing to control the accumulation of virus parasite satellite RNA. (literal)
- Abstract
- Cymbidium ringspot virus (CymRSV) satellite RNA (satRNA) is a parasitic subviral RNA replicon that replicates and accumulates at the cost of its helper virus. This 621-nt satRNA species has no sequence similarity to the helper virus except from a 51-nt long region termed the helper-satellite homology (HSH) region, which essential for satRNA replication. We show that the accumulation of satRNA strongly depends on temperature and on the presence of the helper virus p19 silencing suppressor protein, suggesting that RNA silencing plays a crucial role in satRNA accumulation. We also demonstrate that another member of the Tombusvirus genus, Carnation Italian ringspot virus (CIRV), supports satRNA accumulation at a higher level than CymRSV. Our results suggest that siRNA derived from CymRSV targets satRNA more efficiently than siRNA from CIRV, possibly because of the higher sequence similarity between the HSH regions of the helper and CIRV satRNA. RNA silencing sensor RNA carrying the putative satRNA target site in the HSH region was efficiently cleaved when transiently expressed in CymRSV-infected plants, but not in CIRV-infected plants. Strikingly, replacing the CymRSV HSH box2 sequence with that of CIRV restores satRNA accumulation both at 24 degrees C and in the absence of the p19 suppressor protein. These findings demonstrate the extraordinary adaptation of this virus to its host in terms of harnessing the antiviral silencing response of the plant to control the virus parasite satRNA. (literal)
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