Identification of a RNA silencing suppressor in the genome of Grapevine virus A. (Articolo in rivista)

Type
Label
  • Identification of a RNA silencing suppressor in the genome of Grapevine virus A. (Articolo in rivista) (literal)
Anno
  • 2006-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1099/vir.0.81893-0 (literal)
Alternative label
  • ZHOU Z.SH; DELL'ORCO M.; SALDARELLI P.; TURTURO C.; MINAFRA A.; MARTELLI G.P. (2006)
    Identification of a RNA silencing suppressor in the genome of Grapevine virus A.
    in Journal of general virology (Print)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • ZHOU Z.SH; DELL'ORCO M.; SALDARELLI P.; TURTURO C.; MINAFRA A.; MARTELLI G.P. (literal)
Pagina inizio
  • 2387 (literal)
Pagina fine
  • 2395 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 87 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#descrizioneSinteticaDelProdotto
  • Identificazione di un sopressore del silenziamento genico del genoma di GVA (literal)
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Dipartimento di Protezione delle Piante e Microbiologia Applicata, Universita` degli Studi and Istituto di Virologia Vegetale CNR, Sezione di Bari, Via Amendola 165/A, 70126 Bari, Italy (literal)
Titolo
  • Identification of a RNA silencing suppressor in the genome of Grapevine virus A. (literal)
Abstract
  • Higher plants use post-transcriptional gene silencing (PTGS), an RNA-degradation system, as a defence mechanism against viral infections. To counteract this, plant viruses encode and express PTGS suppressor proteins. Four of the five proteins encoded by the Grapevine virus A (GVA) genome were screened using a green fluorescent protein (GFP)-based transient expression assay, and the expression product of ORF5 (protein p10) was identified as a suppressor of silencing. ORF5 p10 suppressed local and systemic silencing induced by a transiently expressed single-stranded sense RNA. This protein was active towards both a transgene and exogenous GFP mRNAs. Ectopic expression of GVA-ORF5 by a Potato virus X vector enhanced symptom severity. The findings that p10 markedly reduces the levels of small interfering RNAs (siRNAs) and that the recombinant protein is able to bind single-stranded and double-stranded forms of siRNAs and microRNAs, suggest the existence of a potential mechanism of suppression based on RNA sequestering. (literal)
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