Characterization of four viral species belonging to the family potyviridae isolated from Ranunculus asiaticus. (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• Characterization of four viral species belonging to the family potyviridae isolated from Ranunculus asiaticus. (Articolo in rivista) (literal)

Anno

• 2006-01-01T00:00:00+01:00 (literal)

Alternative label

• TURINA, M., CIUFFO, M., LENZI, R., ROSTAGNO, L.,MELA, L ., DERIN, E. PALMANO, S. (2006)
  Characterization of four viral species belonging to the family potyviridae isolated from Ranunculus asiaticus.
  in Phytopathology
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• TURINA, M., CIUFFO, M., LENZI, R., ROSTAGNO, L.,MELA, L ., DERIN, E. PALMANO, S. (literal)

Pagina inizio

• 560 (literal)

Pagina fine

• 566 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 96 (literal)

Rivista

• Phytopathology (Rivista)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• MELA, L. Istituto Regionale per la Floricoltura di Sanremo DERIN, E. Istituto Regionale per la Floricoltura di Sanremo (literal)

Titolo

• Characterization of four viral species belonging to the family potyviridae isolated from Ranunculus asiaticus. (literal)

Abstract

• Four different viral species were isolated from diseased Ranunculus asiaticus plants growing in Imperia Province (Italian Riviera-Liguria Region). Infected plants exhibited mosaic symptoms and growth abnormalities. The viruses were mechanically inoculated to a range of herbaceous hosts and differentiated biologically. Long flexuous particles were present in leaf dip extracts observed by electron microscopy. A general protocol for the amplification of potyvirus genome fragments through reverse transcription-polymerase chain reaction generated products that were cloned and sequenced. Sequence and phylogenetic analysis suggested that three of these isolates could be considered new viral species belonging to the genus Potyvirus. The fourth isolate is a new member of the genus Macluravirus. Purified virus was used as antigen to produce a specific polyclonal antiserum in rabbit; serological features were established through double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA), antigen coated plate (ACP)-ELISA, and western blot analysis. DAS-ELISA was highly specific for each virus isolate, whereas some cross-reactivity was shown in ACP-ELISA and western blot analysis. Aphid transmission by Myzus persicae was demonstrated in a controlled environment for each of the four viral isolates, whereas no transmission through seed was observed. (literal)

Prodotto di

• Interazioni biologiche e molecolari delle piante con virus e agenti patogeni virus-simili (AG.P01.010.001) (Modulo)

Autore CNR

• RICCARDO LENZI (Unità di personale interno)
• SABRINA PALMANO (Unità di personale interno)
• MARINA CIUFFO (Unità di personale interno)
• MASSIMO TURINA (Unità di personale interno)

Insieme di parole chiave

• Keywords of "Characterization of four viral species belonging to the family potyviridae isolated from Ranunculus asiaticus." (Insieme di parole chiave)

Incoming links:

Autore CNR di

• MASSIMO TURINA (Unità di personale interno)
• SABRINA PALMANO (Unità di personale interno)
• MARINA CIUFFO (Unità di personale interno)
• RICCARDO LENZI (Unità di personale interno)

Prodotto

• Interazioni biologiche e molecolari delle piante con virus e agenti patogeni virus-simili (AG.P01.010.001) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Phytopathology (Rivista)

Insieme di parole chiave di

• Keywords of "Characterization of four viral species belonging to the family potyviridae isolated from Ranunculus asiaticus." (Insieme di parole chiave)