Simultaneous detection of nine grapevine viruses by multiplex reverse transcription polymerase chain reaction with coamplification of a plant RNA as internal control. (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Simultaneous detection of nine grapevine viruses by multiplex reverse transcription polymerase chain reaction with coamplification of a plant RNA as internal control. (Articolo in rivista) (literal)

Anno

• 2006-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1094/PHYTO-96-1223 (literal)

Alternative label

• Giorgio GAMBINO; Ivana GRIBAUDO (2006)
  Simultaneous detection of nine grapevine viruses by multiplex reverse transcription polymerase chain reaction with coamplification of a plant RNA as internal control.
  in Phytopathology; American Phytopathological Society, St Paul (Stati Uniti d'America)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Giorgio GAMBINO; Ivana GRIBAUDO (literal)

Pagina inizio

• 1223 (literal)

Pagina fine

• 1229 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 96 (literal)

Rivista

• Phytopathology (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 11 (literal)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Plant Virology Institute CNR (IVV), Grugliasco Unit. Via L. da Vinci 44, I-10095, Grugliasco-TO, Italy. (literal)

Titolo

• Simultaneous detection of nine grapevine viruses by multiplex reverse transcription polymerase chain reaction with coamplification of a plant RNA as internal control. (literal)

Abstract

• A multiplex reverse-transcription polymerase chain reaction (mRT-PCR) was developed for simultaneous detection of nine grapevine viruses: Arabis mosaic virus, Grapevine fanleaf virus, Grapevine virus A, Grapevine virus B, Rupestris stem pitting-associated virus, Grapevine fleck virus, Grapevine leafroll-associated virus -1, -2 and -3, in combination with a plant RNA internal control used as an indicator of the effectiveness of RNA extraction and RT-PCR. Primers were designed from conserved regions of each virus and their specificity was confirmed by sequencing PCR products. Two plant total RNA extraction methods (silica capture and modified RNeasy method) and two RT-PCR systems (one-step and two-step) were evaluated to develop a reliable protocol for mRT-PCR. One to nine fragments specific for the viruses were simultaneously amplified from infected samples and identified by their specific molecular sizes in agarose gel electrophoresis. In the two-step mRT-PCR the detection limits were 10-3 or 10-4 extract dilutions, depending on the virus. Leaves, phloem from dormant cuttings and in vitro plantlets from 103 naturally infected and healthy grapevines were analyzed. The multiplex RT-PCR provided a reliable and rapid method for detecting grapevine viruses from a large number of samples. (literal)

Editore

• American Phytopathological Society (Editore)

Prodotto di

• Agenti patogeni intracellulari e miglioramento genetico e sanitario finalizzato alla valorizzazione del germoplasma viticolo (AG.P04.009.001) (Modulo)

Autore CNR

• IVANA GRIBAUDO (Persona)
• GIORGIO GAMBINO (Persona)

Insieme di parole chiave

• Parole chiave di "Simultaneous detection of nine grapevine viruses by multiplex reverse transcription polymerase chain reaction with coamplification of a plant RNA as internal control." (Insieme di parole chiave)

Incoming links:

Autore CNR di

• IVANA GRIBAUDO (Persona)
• GIORGIO GAMBINO (Persona)

Prodotto

• Agenti patogeni intracellulari e miglioramento genetico e sanitario finalizzato alla valorizzazione del germoplasma viticolo (AG.P04.009.001) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Phytopathology (Rivista)

Editore di

• American Phytopathological Society (Editore)

Insieme di parole chiave di

• Parole chiave di "Simultaneous detection of nine grapevine viruses by multiplex reverse transcription polymerase chain reaction with coamplification of a plant RNA as internal control." (Insieme di parole chiave)