The cauliflower mosaic virion-associated protein is dispensable for viral replication in single cells (Articolo in rivista)

Type
Label
  • The cauliflower mosaic virion-associated protein is dispensable for viral replication in single cells (Articolo in rivista) (literal)
Anno
  • 2002-01-01T00:00:00+01:00 (literal)
Alternative label
  • Kobayashi K. 1, Tsuge S. 2, Stavolone L. 3, Hohn T. 4 (2002)
    The cauliflower mosaic virion-associated protein is dispensable for viral replication in single cells
    in Journal of virology (Print)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Kobayashi K. 1, Tsuge S. 2, Stavolone L. 3, Hohn T. 4 (literal)
Pagina inizio
  • 9457 (literal)
Pagina fine
  • 9464 (literal)
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  • 18 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#descrizioneSinteticaDelProdotto
  • - vedi abstract (literal)
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • 3: Livia Stavolone IVV-Bari 1,4: Friedrich Miescher Institut, Basilea, Svizzera 2: Kyoto Prefectural University, Kyoto, Japan (literal)
Titolo
  • The cauliflower mosaic virion-associated protein is dispensable for viral replication in single cells (literal)
Abstract
  • Cauliflower mosaic virus (CaMV) open reading frame III (ORF III) codes for a virion-associated protein (Vap), which is one of two viral proteins essential for aphid transmission. However, unlike the aphid transmission factor encoded by CaMV ORF II, Vap is also essential for systemic infection, suggesting that it is a multifunctional protein. To elucidate the additional function or functions of Vap, we tested the replication of noninfectious ORF III-defective mutants in transfected turnip protoplasts. PCR and Western blot analyses revealed that CaMV replication had occurred with an efficiency similar to that of wild-type virus and without leading to reversions. Electron microscopic examination revealed that an ORF III frameshift mutant formed normally structured virions. These results demonstrate that Vap is dispensable for replication in single cells and is not essential for virion morphogenesis. Analysis of inoculated turnip leaves showed that the ORF III frameshift mutant does not cause any detectable local infection. These results are strongly indicative of a role for Vap in virus movement. (literal)
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