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In situ localization and tissue distribution of the replication-associated proteins of Cucumber mosaic virus in tobacco and cucumber (Articolo in rivista)

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In situ localization and tissue distribution of the replication-associated proteins of Cucumber mosaic virus in tobacco and cucumber (Articolo in rivista) (literal)

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Cillo F.1, Roberts I.M. 2, Palukaitis P. 3 (2002)
In situ localization and tissue distribution of the replication-associated proteins of Cucumber mosaic virus in tobacco and cucumber
in Journal of virology (Print)
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Questo lavoro aggiunge importanti conoscenze a quanto già noto sulla replicazione di virus vegetali della famiglia Bromoviridae. Lo studio è infatti compiuto con uno strumento molto più sensibile rispetto a quelli usati in precedenza (microscopio elettronico invece che confocale) e si estende a considerare la distribuzione delle proteine associate alla replicasi virale in differenti tessuti, piuttosto che soffermarsi sulla singola cellula dell'ospite. (literal)

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Viene per la prima volta evidenziata, attraverso tecniche di immunodiagnosi ed ibridazione molecolare accoppiate alla microscopia elettronica, la localizzazione subcellulare delle proteine 1a e 2a associate alla replicasi di CMV a livello del tonoplasto. E' anche presa in esame la distribuzione differenziale di CMV-1a e CMV-2a in due diverse piante ospiti, tabacco e cetriolo, ed in diversi tessuti fogliari. Ne emerge la possibile presenza di fattori dell'ospite, che agiscono nel restringere l'accumulo di 1a e 2a in specifici tessuti (fascii vascolari immaturi in tabacco, epidermide e mesofillo in cetriolo). (literal)

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1. CNR, Istituto di Virologia Vegetale, sez. di Bari 2, 3. Scottish Crop Research Institute, Dundee, UK (literal)

Titolo

In situ localization and tissue distribution of the replication-associated proteins of Cucumber mosaic virus in tobacco and cucumber (literal)

Abstract

-The replication-associated proteins encoded by Cucumber mosaic virus (CMV), the 1a and 2a proteins, were detected by immunogold labeling in two host species of this virus, tobacco (Nicotiana tabacum) and cucumber (Cucumis sativus). In both hosts, the 1a and 2a proteins colocalized predominantly to the vacuolar membranes, the tonoplast. While plus-strand CMV RNAs were found distributed throughout the cytoplasm by in situ hybridization, minus-strand CMV RNAs were barely detectable but were found associated with the tonoplast. In both cucumber and tobacco, 2a protein was detected at higher densities than 1a protein. The 1a and 2a proteins also showed quantitative differences with regard to tissue distributions in tobacco and cucumber. About three times as much 2a protein was detected in CMV-infected cucumber tissues as in CMV-infected tobacco tissues. In tobacco, high densities of these proteins were observed only in vascular bundle cells of minor veins. In contrast, in cucumber, high densities of 1a and 2a proteins were observed in mesophyll cells, followed by epidermis cells, with only low levels being observed in vascular bundle cells. Differences were also observed in the distributions of 2a protein and capsid protein in vascular bundle cells of the two host species. These observations may represent differences in the relative rates of tissue infection in different hosts or differences in the extent of virus replication in vascular tissues of different hosts. (literal)

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