Complete nucleotide sequence and genome organization of grapevine fleck virus. (Articolo in rivista)

Type
Label
  • Complete nucleotide sequence and genome organization of grapevine fleck virus. (Articolo in rivista) (literal)
Anno
  • 2001-01-01T00:00:00+01:00 (literal)
Alternative label
  • Sabanadzovic S. 1, Abou Ghanem-Sabanadzovic N. 2, Saldarelli P. 3, Martelli G P. 4. (2001)
    Complete nucleotide sequence and genome organization of grapevine fleck virus.
    in Journal of general virology (Print)
    (literal)
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  • Sabanadzovic S. 1, Abou Ghanem-Sabanadzovic N. 2, Saldarelli P. 3, Martelli G P. 4. (literal)
Pagina inizio
  • 2009 (literal)
Pagina fine
  • 2015 (literal)
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  • ... (literal)
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  • 82 (literal)
Rivista
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  • Si descrive la sequenza completa dell'RNA genomico del virus della maculatura infettiva della vite (GFkV).La struttura e l'organizzazione del genoma sono molto simili a quella di membri dei generi Tymovirus e Marafivirus.Comunque differenze biologiche, epidemiologiche, citopatologiche e molecolari inducono ad una diversa classificazione tassonomica di GFkV. (literal)
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • 1,2 Istituto Agronomico Mediterraneo, Valenzano, Bari.3. Istituto di Virologia Vegetale-Sezione di Bari.4. Dipartimento di Protezione delle Piante e Microbiologia Applicata, Università di Bari (literal)
Titolo
  • Complete nucleotide sequence and genome organization of grapevine fleck virus. (literal)
Abstract
  • The complete nucleotide sequence of Grapevine fleck virus (GFkV) genomic RNA was determined. The genome is 7564 nt in size, excluding the 3'-terminal poly(A) tail, is characterized by an extremely high cytosine content (ca. 50%), and contains four putative open reading frames and untranslated regions of 291 and 35 nt at the 5' and 3' ends, respectively. ORF 1 potentially encodes a 215.4 kDa polypeptide (p215), which has the conserved motifs of replication-associated proteins of positive-strand RNA viruses. ORF 2 encodes a 24.3 kDa polypeptide (p24) identified as the coat protein. ORFs 3 and 4 are located at the extreme 3' end of the viral genome and encode proline-rich proteins of 31.4 kDa (p31) and 15.9 kDa (p16), respectively, of unknown function. Phylogenetic analysis of the viral replicase and coat protein genes showed that GFkV is related to members of the Tymovirus and Marafivirus genera. Two subgenomic RNAs were present in the GFkV preparations as ascertained by molecular hybridization. The genome organization of GFkV resembles to some extent that of tymoviruses and marafiviruses. However, differences in the biological and epidemiological behaviour, cytopathology and molecular properties (i.e. size of genomic RNA and coat protein, and number of ORFs) support the notion that GFkV is a separate virus belonging in a new genus. (literal)
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