Tomato yellow leaf curl Sardinia virus rep-derived resistance to homologous and heterologous geminiviruses occurs by different mechanisms and is overcome if virus-mediated transgene silencing is activated. (Articolo in rivista)

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  • Tomato yellow leaf curl Sardinia virus rep-derived resistance to homologous and heterologous geminiviruses occurs by different mechanisms and is overcome if virus-mediated transgene silencing is activated. (Articolo in rivista) (literal)
Anno
  • 2003-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1128/JVI.77.12.6785-6798.2003 (literal)
Alternative label
  • Lucioli A., Noris E., Brunetti A., Tavazza R., Ruzza V., Castillo A.G., Bejarano E.R., Accotto G.P.,Tavazza M. (2003)
    Tomato yellow leaf curl Sardinia virus rep-derived resistance to homologous and heterologous geminiviruses occurs by different mechanisms and is overcome if virus-mediated transgene silencing is activated.
    in Journal of virology (Print)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Lucioli A., Noris E., Brunetti A., Tavazza R., Ruzza V., Castillo A.G., Bejarano E.R., Accotto G.P.,Tavazza M. (literal)
Pagina inizio
  • 6785 (literal)
Pagina fine
  • 6798 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#altreInformazioni
  • Study concerning the mechanism of action of a mutated viral protein expressed in transgenic plants, conferring virus resistance. By a detailed analysis two mechanisms of activity, one against the homologous virus from which the transgene originated, one acting agaist a closely related virus have been identified. Moreover, the ability of a virus to silence the expression of a virus-derived gene expressed in plants is described for the first time. This has strong implications on the ability to produce virus resistant plants (literal)
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  • http://jvi.asm.org/content/77/12/6785.full.pdf+html (literal)
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  • 77 (literal)
Rivista
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  • 13 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
  • 12 (literal)
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  • L'espressione della proteina Rep210 conferisce resistanza a TYLCSV, mediante inibizione della replicazione del virus. E' stato studiato approfonditamente il meccanismo biologico e, utilizzando mutanti di delezione, espressione transiente in protoplasti, saggi di espressione con geni reporter, tecnica del doppio ibrido in lievito e infine utilizzando piante transgeniche si è arrivati alle seguenti conclusioni. L'inibizione della replicazione virale da parte della Rep-210 si manifesta attraverso due meccanismi biologici: nel caso della infezione da parte del virus omologo (TYLCSV) la Rep210 inibisce la trascrizione del corrispondente gene virale, la proteina Rep. Nel caso di infezione da parte di un virus simile ma distinto (TYLCV-PT), la Rep-210 interferisce con la proteina virale Rep corrispondente, formando del complessi disfunzionali. (literal)
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • NE, AGP: Istituto di Virologia Vegetale, CNR, Strada delle Cacce 73, 10135 Torino, Italy; LA, TM, TR, RV, BA: ENEA, CR Casaccia, Via Anguillarese 301, 00060 Santa Maria di Galeria ROMA; CAG, BER: Departamento de Biología Celular, Genética y Fisiología, Universidad de Málaga, Campus de Teatinos. 29071 Málaga (Spain) (literal)
Titolo
  • Tomato yellow leaf curl Sardinia virus rep-derived resistance to homologous and heterologous geminiviruses occurs by different mechanisms and is overcome if virus-mediated transgene silencing is activated. (literal)
Abstract
  • The replication-associated protein (Rep) of geminiviruses is involved in several biological processes brought about by the presence of distinct functional domains. Recently, we have exploited the multifunctional character of the Tomato yellow leaf curl Sardinia virus (TYLCSV) Rep to develop a molecular interference strategy to impair TYLCSV infection. We showed that transgenic expression of its N-terminal 210 amino acids (Rep-210) confers resistance to the homologous virus by inhibiting viral transcription and replication. We have now used biochemical and transgenic approaches to carry out a fuller investigation of the molecular resistance mechanisms in transgenic plants expressing Rep-210. We show that Rep-210 confers resistance through two distinct molecular mechanisms, depending on the challenging virus. Resistance to the homologous virus is achieved by the ability of Rep-210 to tightly inhibit C1 gene transcription, while that to heterologous virus is due to the interacting property of the Rep-210 oligomerization domain. Furthermore, we present evidence that in Rep-210-expressing plants, the duration of resistance is related to the ability of the challenging virus to shut off transgene expression by a posttranscriptional homology-dependent gene silencing mechanism. A model of Rep-210-mediated geminivirus resistance that takes transgene- and virus-mediated mechanisms into account is proposed. (literal)
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